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ZFIN ID: ZDB-FIG-060908-2
Cheng et al., 2006 - HNF factors form a network to regulate liver-enriched genes in zebrafish. Developmental Biology   294(2):482-496 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Adult

Fig. 1 Classification of liver-enriched genes in zebrafish. RNA samples were extracted from the dissected liver and liver-free body, respectively. In panel A, RNA samples from 3 dpf embryos were also included. Probes were derived from EST clones or gene fragments cloned in plasmid vector via RT-PCR using gene-specific primers (Table 1; Supplementary Table 4). RNA hybridization blots were arranged according to the descending ratio of expression levels of liver vs. liver-free body (as indicated in the bracket). We arbitrarily grouped this set of genes according to the ratio of expression level. Genes with ratios more than 6.9 are considered as liver-specific genes, ratios between 6.9 and 2 as highly-liver-enriched genes and ratios less than 2 as moderately-liver-enriched genes. Clone identification is shown on the right side. Hybridization intensity was quantified using software Molecular Analyst (Bio-rad). B, liver-free body; L, liver; E, 3 dpf embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
a1cf WT standard conditions Protruding-mouth to Day 5 liver ISH
Adult liver RTPCR
Adult whole organism RTPCR
acaa1 WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
adh8a WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
agt WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
ahcy WT standard conditions Protruding-mouth whole organism RTPCR
Protruding-mouth to Day 5 intestine ISH
Protruding-mouth to Day 5 liver ISH
Protruding-mouth to Day 5 pancreas ISH
Adult liver RTPCR
Adult whole organism RTPCR
ahsg1 WT standard conditions Adult liver RTPCR
Adult whole organism RTPCR
aldob WT standard conditions Protruding-mouth whole organism RTPCR
Protruding-mouth to Day 5 intestine ISH
Protruding-mouth to Day 5 liver ISH
Protruding-mouth to Day 5 pancreas ISH
Adult liver RTPCR
Adult whole organism RTPCR
ambp WT standard conditions Adult liver RTPCR
Adult whole organism RTPCR
apoa2 WT standard conditions Protruding-mouth whole organism RTPCR
Protruding-mouth to Day 5 liver ISH
Adult liver RTPCR
Adult whole organism RTPCR
apoba WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
apoc1 WT standard conditions Protruding-mouth whole organism RTPCR
Protruding-mouth to Day 5 liver ISH
Adult liver RTPCR
apoc2 WT standard conditions Protruding-mouth whole organism RTPCR
Protruding-mouth to Day 5 intestine ISH
Protruding-mouth to Day 5 liver ISH
Protruding-mouth to Day 5 pancreas ISH
Adult liver RTPCR
Adult whole organism RTPCR
apom WT standard conditions Protruding-mouth whole organism RTPCR
Protruding-mouth to Day 5 liver ISH
Adult liver RTPCR
Adult whole organism RTPCR
bckdhbl WT standard conditions Protruding-mouth to Day 5 liver ISH
bhmt WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
c8a WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Protruding-mouth to Day 5 liver ISH
Adult liver RTPCR
Adult whole organism RTPCR
c8gl WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
c9 WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
cbln10 WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Protruding-mouth to Day 5 liver ISH
Adult liver RTPCR
cfb WT standard conditions Adult liver RTPCR
Adult whole organism RTPCR
chrna5 WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
cp WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
cpn1 WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
cyp2aa6 WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Protruding-mouth to Day 5 liver ISH
Adult liver RTPCR
cyp2ad2 WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
cyp2x12 WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
cyp24a1 WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
dhrs9 WT standard conditions Protruding-mouth intestine RTPCR
Protruding-mouth liver RTPCR
Protruding-mouth Not Detected pancreas RTPCR
Protruding-mouth Not Detected whole organism RTPCR
Day 4 to Day 5 intestine ISH
Day 4 to Day 5 liver ISH
Day 4 to Day 5 Not Detected pancreas ISH
Adult liver RTPCR
Adult whole organism RTPCR
dio2 WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Adult liver RTPCR
dync1li1 WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Adult liver RTPCR
esr1 WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Adult liver RTPCR
esr2b WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
f2 WT standard conditions Protruding-mouth whole organism RTPCR
Protruding-mouth to Day 5 liver ISH
Adult liver RTPCR
fabp10a WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Adult liver RTPCR
fads2 WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
fbp1b WT standard conditions Protruding-mouth whole organism RTPCR
Protruding-mouth to Day 5 intestine ISH
Protruding-mouth to Day 5 liver ISH
Protruding-mouth to Day 5 Not Detected pancreas ISH
Adult liver RTPCR
Adult whole organism RTPCR
fetub WT standard conditions Protruding-mouth whole organism RTPCR
Protruding-mouth to Day 5 liver ISH
Adult liver RTPCR
Adult whole organism RTPCR
fga WT standard conditions Protruding-mouth whole organism RTPCR
Protruding-mouth to Day 5 liver ISH
Adult liver RTPCR
Adult whole organism RTPCR
fgg WT standard conditions Protruding-mouth whole organism RTPCR
Protruding-mouth to Day 5 liver ISH
Adult liver RTPCR
Adult whole organism RTPCR
fn1b WT standard conditions Adult liver RTPCR
Adult whole organism RTPCR
foxa3 WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
fxyd1 WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
gatm WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Protruding-mouth to Day 5 intestine ISH
Protruding-mouth to Day 5 liver ISH
Protruding-mouth to Day 5 pancreas ISH
Adult liver RTPCR
gchfr WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Adult liver RTPCR
gdal WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Adult liver RTPCR
Adult Not Detected whole organism RTPCR
gpt2l WT standard conditions Protruding-mouth to Day 5 liver ISH
Adult liver RTPCR
gstp1 WT standard conditions Protruding-mouth whole organism RTPCR
Protruding-mouth to Day 5 intestine ISH
Protruding-mouth to Day 5 liver ISH
Protruding-mouth to Day 5 Not Detected pancreas ISH
Adult liver RTPCR
Adult whole organism RTPCR
hnf4a WT standard conditions Protruding-mouth whole organism RTPCR
Protruding-mouth to Day 5 intestine ISH
Protruding-mouth to Day 5 liver ISH
Protruding-mouth to Day 5 Not Detected pancreas ISH
Adult liver RTPCR
Adult whole organism RTPCR
hp WT standard conditions Protruding-mouth whole organism RTPCR
Protruding-mouth to Day 5 liver ISH
Adult liver RTPCR
hpda WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
hpxa WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
igfbp2a WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Protruding-mouth to Day 5 liver ISH
Adult liver RTPCR
itih2 WT standard conditions Protruding-mouth to Day 5 liver ISH
Adult liver RTPCR
Adult whole organism RTPCR
kng1 WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
ldhbb WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
lipca WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
lpl WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
mat1a WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
med12 WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
mpx WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Adult liver RTPCR
Adult Not Detected whole organism RTPCR
mst1 WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
mycla WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
nots WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
nupr1b WT standard conditions Protruding-mouth whole organism RTPCR
Protruding-mouth to Day 5 intestine ISH
Protruding-mouth to Day 5 liver ISH
Protruding-mouth to Day 5 pancreas ISH
Adult liver RTPCR
Adult whole organism RTPCR
olfm1b WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Protruding-mouth to Day 5 liver ISH
Adult liver RTPCR
pklr WT standard conditions Protruding-mouth whole organism RTPCR
Protruding-mouth to Day 5 intestine ISH
Protruding-mouth to Day 5 liver ISH
Protruding-mouth to Day 5 Not Detected pancreas ISH
Adult liver RTPCR
Adult whole organism RTPCR
plg WT standard conditions Protruding-mouth whole organism RTPCR
Protruding-mouth to Day 5 liver ISH
Adult liver RTPCR
Adult whole organism RTPCR
pnp4b WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Adult liver RTPCR
proca WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
rbp4 WT standard conditions Protruding-mouth whole organism RTPCR
Protruding-mouth to Day 5 liver ISH
Adult liver RTPCR
Adult whole organism RTPCR
rcn3 WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Adult liver RTPCR
rpl5a WT standard conditions Protruding-mouth whole organism RTPCR
Protruding-mouth to Day 5 intestine ISH
Protruding-mouth to Day 5 pancreas ISH
Adult liver RTPCR
Adult whole organism RTPCR
rps6 WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
sat2a WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
scp2a WT standard conditions Adult liver RTPCR
sdr16c5b WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
serpina1l WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
serpina7 WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
serpinc1 WT standard conditions Adult liver RTPCR
Adult whole organism RTPCR
serpind1 WT standard conditions Protruding-mouth whole organism RTPCR
Protruding-mouth to Day 5 liver ISH
Adult liver RTPCR
serpinf2a WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Adult liver RTPCR
serping1 WT standard conditions Adult liver RTPCR
Adult whole organism RTPCR
sid4 WT standard conditions Protruding-mouth liver RTPCR
Protruding-mouth Not Detected whole organism RTPCR
Adult liver RTPCR
slc1a3a WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Adult liver RTPCR
slc38a4 WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Adult liver RTPCR
sox4a WT standard conditions Adult liver RTPCR
Adult whole organism RTPCR
tfa WT standard conditions Protruding-mouth whole organism RTPCR
Protruding-mouth to Day 5 liver ISH
Adult liver RTPCR
Adult whole organism RTPCR
tgfbi WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
tprb WT standard conditions Protruding-mouth whole organism RTPCR
Day 4 to Day 5 liver ISH
Adult liver RTPCR
Adult whole organism RTPCR
tpt1 WT standard conditions Protruding-mouth whole organism RTPCR
Protruding-mouth to Day 5 liver ISH
Adult liver RTPCR
Adult whole organism RTPCR
uox WT standard conditions Protruding-mouth whole organism RTPCR
Protruding-mouth to Day 5 liver ISH
Adult liver RTPCR
Adult whole organism RTPCR
vcanb WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
vtg2 WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Adult liver RTPCR
vtnb WT standard conditions Protruding-mouth whole organism RTPCR
Protruding-mouth to Day 5 liver ISH
Adult liver RTPCR
Adult whole organism RTPCR
wu:fb58h05 WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Protruding-mouth to Day 5 liver ISH
Adult liver RTPCR
Adult whole organism RTPCR
wu:fc26d05 WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
wu:fd55c06 WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Adult liver RTPCR
wu:fi15h08 WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Adult liver RTPCR
zar1 WT standard conditions Protruding-mouth to Day 5 liver ISH
Adult liver RTPCR
Adult whole organism RTPCR
zgc:112265 WT standard conditions Adult liver RTPCR
zgc:123103 WT standard conditions Protruding-mouth whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
zgc:153921 WT standard conditions Protruding-mouth Not Detected whole organism RTPCR
Adult liver RTPCR
Adult whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 294(2), Cheng, W., Guo, L., Zhang, Z., Soo, H.M., Wen, C., Wu, W., and Peng, J., HNF factors form a network to regulate liver-enriched genes in zebrafish, 482-496, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.