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ZFIN ID: ZDB-FIG-120601-53
Chu et al., 2012 - Overexpression of akt1 enhances adipogenesis and leads to lipoma formation in zebrafish. PLoS One   7(5):e36474 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 7 Deregulation of myogenesis-, skeletogenesis-, adipogenesis-, adipocytokine- and inflammation-related genes in Tg(krt4:Hsa.myrAkt1)cy18.

Comparison of relative expression level of myogenesis/skeletogenesis- (A), adipogenesis- (B), adipocytokine and inflammatory-related (C) gene transcripts between WT and Tg assayed by real-time RT-PCR. Stars labeled above the error bars indicate significant differences as tested by Student’s t-test.*p<0.05, **p<0.01, and ***p<0.001.Visualization of the inflammatory response (red dotted line) in the tail region of both Tg(mpx:GFP)i113 (D) and double transgenic progeny derived from Tg(mpx:GFP)i113 and Tg(krt4:Hsa.myrAkt1)cy18 crossing (E).WT, wild type; Tg, Tg(krt4:Hsa.myrAkt1)cy18.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
adipoqa AB standard conditions Adult whole organism RTPCR
cy18Tg standard conditions Adult caudal fin RTPCR
adipoqb AB standard conditions Adult whole organism RTPCR
cy18Tg standard conditions Adult caudal fin RTPCR
adipor1a AB standard conditions Adult whole organism RTPCR
cy18Tg standard conditions Adult caudal fin RTPCR
adipor1b AB standard conditions Adult whole organism RTPCR
cy18Tg standard conditions Adult caudal fin RTPCR
adipor2 AB standard conditions Adult whole organism RTPCR
cy18Tg standard conditions Adult whole organism RTPCR
cebpa AB standard conditions Adult whole organism RTPCR
cy18Tg standard conditions Adult whole organism RTPCR
col2a1a AB standard conditions Adult caudal fin RTPCR
cy18Tg standard conditions Adult whole organism RTPCR
fabp11a AB standard conditions Adult whole organism RTPCR
cy18Tg standard conditions Adult caudal fin RTPCR
fabp11b AB standard conditions Adult whole organism RTPCR
cy18Tg standard conditions Adult caudal fin RTPCR
il1b AB standard conditions Adult whole organism RTPCR
cy18Tg standard conditions Adult caudal fin RTPCR
lepr AB standard conditions Adult whole organism RTPCR
cy18Tg standard conditions Adult caudal fin RTPCR
lpin1 AB standard conditions Adult whole organism RTPCR
cy18Tg standard conditions Adult caudal fin RTPCR
lpl AB standard conditions Adult whole organism RTPCR
cy18Tg standard conditions Adult caudal fin RTPCR
mgp AB standard conditions Adult caudal fin RTPCR
cy18Tg standard conditions Adult caudal fin RTPCR
mmp2 AB standard conditions Adult whole organism RTPCR
cy18Tg standard conditions Adult caudal fin RTPCR
mmp9 AB standard conditions Adult whole organism RTPCR
cy18Tg standard conditions Adult caudal fin RTPCR
myf5 AB standard conditions Adult caudal fin RTPCR
cy18Tg standard conditions Adult caudal fin RTPCR
myf6 AB standard conditions Adult caudal fin RTPCR
cy18Tg standard conditions Adult caudal fin RTPCR
mylpfa AB standard conditions Adult caudal fin RTPCR
cy18Tg standard conditions Adult caudal fin RTPCR
myod1 AB standard conditions Adult caudal fin RTPCR
cy18Tg standard conditions Adult caudal fin RTPCR
myog AB standard conditions Adult caudal fin RTPCR
cy18Tg standard conditions Adult caudal fin RTPCR
pparg AB standard conditions Adult whole organism RTPCR
cy18Tg standard conditions Adult caudal fin RTPCR
runx2a AB standard conditions Adult caudal fin RTPCR
cy18Tg standard conditions Adult caudal fin RTPCR
runx2b AB standard conditions Adult caudal fin RTPCR
cy18Tg standard conditions Adult caudal fin RTPCR
scd AB standard conditions Adult whole organism RTPCR
cy18Tg standard conditions Adult caudal fin RTPCR
srebf1 AB standard conditions Adult whole organism RTPCR
cy18Tg standard conditions Adult whole organism RTPCR
srebf2 AB standard conditions Adult whole organism RTPCR
cy18Tg standard conditions Adult whole organism RTPCR
tnfa AB standard conditions Adult whole organism RTPCR
cy18Tg standard conditions Adult caudal fin RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
cy18Tg; i113Tg standard conditions Adult post-vent region inflammatory, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One