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ZFIN ID: ZDB-FIG-120601-47
Chu et al., 2012 - Overexpression of akt1 enhances adipogenesis and leads to lipoma formation in zebrafish. PLoS One   7(5):e36474 Full text @ PLoS One
Anatomical Terms:
Stage Range: Protruding-mouth to Adult

Fig. 1 Generation of Tg(krt4:Hsa.myrAkt1)cy18.

(A) The schematic diagram in the upper panel shows the configuration of the pDestTol2CG2-krt4-myrAkt1-IRES-EGFP-pA plasmid used to generate Tg(krt4:Hsa.myrAkt1)cy18. The parental vector of pDestTol2CG2, which contains cmlc2-EGFP-pA mini-gene, helps transgenic progeny to express green fluorescent protein in the heart. The lower panel illustrates the domain structure of the wild-type and constitutively active form of human Akt1. (B) Western blot analysis of protein lysate extracted from adult tail fins shows that the exogenous human myrAkt1 (indicated by star) is only detectable in Tg(krt4:Hsa.myrAkt1)cy18. β-actin served as a loading control. Whole-mount immunostaining of wild-type (C) or Tg(krt4:Hsa.myrAkt1)cy18(E) embryos aged 3 days post-fertilization using pan-Akt antibody. The yolk sac region highlighted by a dotted line in C and E is magnified in E and F, respectively. PH, Pleckstrin homology; RD, regulation domain; Myr, myristylation signal; WT, wild type; Tg, Tg(krt4:Hsa.myrAkt1)cy18. Scale bar = 100 μm.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-akt WB AB standard conditions Adult caudal fin
WB cy18Tg standard conditions Adult caudal fin
Ab6-akt WB AB standard conditions Protruding-mouth Not Detected integument
WB cy18Tg standard conditions Protruding-mouth integument
Phenotype Details No data available
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One