Fig. 7 of Nimura et al., 2019
Defects in the migration and polarity of PCs in reln mutants. Sagittal sections of the brain from WT (A-C, n= 4) and relnΔ7/Δ7 (D-H, n= 4) 15-dpf larvae, and from WT (I, n= 3) and relnΔ7/Δ7 (J, n= 3) 30-dpf fish, were stained with anti-Pvalb7 (magenta) and anti-Vglut1 (green) antibodies, and Hoechst (cyan). Typical cerebellum images are shown. The ventral limit of the cerebellum is indicated by a dotted line. (Ba, Ca, Da, Ea, Fa, Ga, Ha) High magnification images of the PCs marked by “a” in B–H. The Pvalb7 and Vglut1-double positive region marks the ML. Migrating PCs were detected in the GCL in WT, and they extended a neurite (primary dendrite) toward the pial side (Ba, Ca, n= 4). Many of the migrating cells extended one or multiple neurites in aberrant directions in the relnΔ7/Δ7mutants (Ea, Fa, Ga, Ha, n= 4). At 30 dpf, most of the PCs had reached the PCL in WT (I), whereas many ectopic PCs were detected in the GCL in relnΔ7/Δ7 mutants (J, n= 3). The abbreviations are described in the legend for Fig. 1. Scale bars: 20 μm in A (applies to A-H); 100 μm in I (applies to I-J).