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ZFIN ID: ZDB-FIG-120601-48
Chu et al., 2012 - Overexpression of akt1 enhances adipogenesis and leads to lipoma formation in zebrafish. PLoS One   7(5):e36474 Full text @ PLoS One
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 2 Tg(krt4:Hsa.myrAkt1)cy18 fish display skin hypertrophy and hyperplasia at the embryonic stage.

Microscopic pictures of skin epidermis near the head regions (A, B), pericardial cavity and yolk sac (C, D), and tail fin (E, F) of either WT (A, C, E) or Tg(krt4:Hsa.myrAkt1)cy18 (B, D, F) at 72 hpf. At 120 hpf, the protruding appearance of skin covering the head (H) and yolk sac (J) was more pronounced in Tg than their wild-type siblings (G, I). Skin histology of WT (K, M) and Tg(L, N) embryos at 120 hpf. Cross sections through the eye position are stained with hematoxylin and eosin. The areas highlighted by a dotted line in K and L are magnified in M and N, respectively. Quantitative comparison of skin thickness (O) and area (P) between WT (black bar) and Tg (red bar). (Q, R) Quantitative comparison of skin density between Tg(krt4:nlsEGFP)cy34 and double transgenics of Tg(krt4:nlsEGFP)cy34;Tg(krt4:Hsa.myrAkt1)cy18 at 120 hpf. ***p<0.001. Data were analyzed by Student’s t-test and are shown as mean ±SEM. hpf, hour post-fertilization; WT, wild type; Tg, Tg(krt4:Hsa.myrAkt1)cy18.

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
cy18Tg standard conditions Protruding-mouth integument hyperplastic, abnormal
Protruding-mouth integument hypertrophic, abnormal
Day 5 integument hyperplastic, abnormal
Day 5 integument hypertrophic, abnormal
Day 5 integument increased area, abnormal
Day 5 integument increased thickness, abnormal
cy18Tg; cy34Tg standard conditions Day 5 integument has extra parts of type cell, abnormal
Day 5 integument hyperplastic, abnormal
cy34Tg standard conditions Day 5 integument size, normal
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One