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ZFIN ID: ZDB-FIG-120601-50
Chu et al., 2012 - Overexpression of akt1 enhances adipogenesis and leads to lipoma formation in zebrafish. PLoS One   7(5):e36474 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage Range: Days 7-13 to Adult

Fig. 4 Tg(krt4:Hsa.myrAkt1)cy18 fish display obese phenotype at the adult stage.

Comparison of the conditional factor between WT (black) and Tg (red) for either male (A) or female (B) fish aged from 3- to 5-month old. Error bars labeled with stars indicate significant differences as tested by Student’s t-test. Oil Red O stains of fish from either wild-type (C), F0 founder carrying krt4:myrAkt1transgene (D), F1 Tg aged at 3 mpf (E), and F1 Tg aged at 12 mpf (F). Nile red vital staining reveals the lipid accumulation in WT and Tg aged at 7 dpf (G and H), 14 dpf (I and J), 21 dpf (K and L), 28 dpf (M and N) and 35 dpf (O and P). Arrows indicate nile red-positive lipids.WT, wild type; Tg, Tg(krt4:Hsa.myrAkt1)cy18; dpf, day post-fertilization; mpf, month post-fertilization.

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
cy18Tg standard conditions Days 7-13 adipose tissue volume, normal
Days 14-20 adipose tissue volume, normal
Days 21-29 pleuroperitoneal cavity adipose tissue increased volume, abnormal
Days 30-44 pleuroperitoneal cavity adipose tissue increased volume, abnormal
Adult caudal fin adipose tissue mislocalised, abnormal
Adult hypodermis adipose tissue mislocalised, abnormal
Adult pleuroperitoneal cavity adipose tissue increased volume, abnormal
Adult positive regulation of growth rate increased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One