|ZFIN ID: ZDB-FIG-120601-50|
|Stage Range:||Days 7-13 to Adult|
Fig. 4 Tg(krt4:Hsa.myrAkt1)cy18 fish display obese phenotype at the adult stage.
Comparison of the conditional factor between WT (black) and Tg (red) for either male (A) or female (B) fish aged from 3- to 5-month old. Error bars labeled with stars indicate significant differences as tested by Student’s t-test. Oil Red O stains of fish from either wild-type (C), F0 founder carrying krt4:myrAkt1transgene (D), F1 Tg aged at 3 mpf (E), and F1 Tg aged at 12 mpf (F). Nile red vital staining reveals the lipid accumulation in WT and Tg aged at 7 dpf (G and H), 14 dpf (I and J), 21 dpf (K and L), 28 dpf (M and N) and 35 dpf (O and P). Arrows indicate nile red-positive lipids.WT, wild type; Tg, Tg(krt4:Hsa.myrAkt1)cy18; dpf, day post-fertilization; mpf, month post-fertilization.
|cy18Tg||standard conditions||Days 7-13||adipose tissue volume, normal|
|Days 14-20||adipose tissue volume, normal|
|Days 21-29||pleuroperitoneal cavity adipose tissue increased volume, abnormal|
|Days 30-44||pleuroperitoneal cavity adipose tissue increased volume, abnormal|
|Adult||caudal fin adipose tissue mislocalised, abnormal|
|Adult||hypodermis adipose tissue mislocalised, abnormal|
|Adult||pleuroperitoneal cavity adipose tissue increased volume, abnormal|
|Adult||positive regulation of growth rate increased process quality, abnormal|
|Acknowledgments:||ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One|