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ZFIN ID: ZDB-FIG-120601-49
Chu et al., 2012 - Overexpression of akt1 enhances adipogenesis and leads to lipoma formation in zebrafish. PLoS One   7(5):e36474 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 5

Fig. 3 The Akt downstream genes are activated in Tg(krt4:Hsa.myrAkt1)cy18.

(A) Western blot analysis of protein lysate extracted from adult tail fins showing that many downstream targets of Akt1 are over-phosphorylated in Tg. β-actin served as a loading control. (B-E) MO knock-down experiment to evaluate whether the skin hypertrophy phenotype can be rescued when Akt activity ceases. Fertilized eggs collected from Tg outcross were injected with either control MO (B), mTOR MO (C), 70S6K MO (D), or mTOR+70S6K MOs (E). The skin hypertrophic appearance was scored at 120 hpf and compared quantitatively (F). MO, morpholino; WT, wild type; Tg, Tg(krt4:Hsa.myrAkt1)cy18; Different letters above the error bars indicate significant differences which tested by one-way ANOVA with Tukey’s pair-wise comparison method. hpf, hour post-fertilization.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-gsk3 WB AB standard conditions Adult caudal fin
WB cy18Tg standard conditions Adult caudal fin
Ab1-mtor WB AB standard conditions Adult caudal fin
WB cy18Tg standard conditions Adult caudal fin
Ab10-rps6k WB AB standard conditions Adult caudal fin
WB cy18Tg standard conditions Adult caudal fin
Phenotype Details
Fish Conditions Stage Phenotype
cy18Tg control Day 5 integument hypertrophic, abnormal
cy18Tg + MO1-mtor standard conditions Day 5 integument size, normal
cy18Tg + MO1-mtor + MO1-rps6ka3a standard conditions Day 5 integument size, normal
cy18Tg + MO1-rps6ka3a standard conditions Day 5 integument size, normal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One