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ZIRC
ZFIN ID: ZDB-FIG-120601-52
Chu et al., 2012 - Overexpression of akt1 enhances adipogenesis and leads to lipoma formation in zebrafish. PLoS One   7(5):e36474 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Term:
Stage: Adult

Fig. 6 Detection of the ectopic expression of myrAkt1 transgene in Tg(krt4:Hsa.myrAkt1)cy18.

(A) Schematic diagram showing the chromosomal integration site in Tg. (B) Relative expression level of myrAkt1 transgene among different tissues assayed by real-time RT-PCR. Stars above the error bars indicate significant differences as tested by Student’s t-test (**p<0.01, and ***p<0.001). The detection of the expression of a Akt downstream target of phospho-mTOR in the adipose tissues of WT (C) and Tg (D) by immunohistochemistry. WT, wild type; Tg, Tg (krt4:Hsa.myrAkt1)cy18.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-mtor IHC AB standard conditions Adult adipose tissue
IHC cy18Tg standard conditions Adult adipose tissue
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One