This material is from the 4th edition of The Zebrafish Book. The 5th edition is available in print and within the ZFIN Protocol Wiki.

THE ZEBRAFISH BOOK
A guide for the laboratory use of zebrafish Danio* (Brachydanio) rerio

by Monte Westerfield, Institute of Neuroscience, University of Oregon

*Older literature referred to zebrafish as Brachydanio rerio. Recent work (Meyer et al., Proc. Roy. Soc. Lond 252:231-236, 1993) and a consensus vote at the 1993 Zebrafish Meeting at the Cold Spring Harbor Laboratory suggest that Danio rerio should now be used. 


   George Streisinger

This book is dedicated to the late George Streisinger
who first began the study of zebrafish in Eugene and
whose insight and gentle support got us all started.
Adapted for the Web by ZFIN. Please address corrections and additions to: Monte Westerfield, Institute of Neuroscience, University of Oregon, Eugene, OR 97403. Copyright © 1993 by Monte Westerfield. 4th Edition, 2000. 5th Edition, 2007.
Citing this information: Westerfield, M. (2000). The zebrafish book. A guide for the laboratory use of zebrafish (Danio rerio). 4th ed., Univ. of Oregon Press, Eugene.
Hardcopies of the 4th edition of The Zebrafish Book can be obtained for a nominal fee from the Zebrafish International Resource Center, 5274 University of Oregon, Eugene, OR 97403 USA; fax: 541-346-6151.

CONTENTS:

(Click chapter title for contents)
  1. GENERAL METHODS FOR ZEBRAFISH CARE
  2. BREEDING
  3. EMBRYONIC AND LARVAL CULTURE
  4. MICROSCOPIC OBSERVATIONS
  5. CELLULAR METHODS
  6. DISSOCIATED CELL CULTURE
  7. GENETIC METHODS
  8. HISTOLOGICAL METHODS
  9. MOLECULAR METHODS
  10. RECIPES