Ca2+ influx occurs predominantly through mechanosensitive channels. (A) xy-trajectory plot of cells exposed to 10µM Gd3+ for 1hour. Scale bar: 200µm. (B) Graph from transient shear stress applied to keratinocyte sheets indicates a transient increase in whole-cell [Ca2+]i during application of fluid flow (n = 3 cell sheets). (C) Whole-cell voltage clamp recording of a migrating keratinocyte in fish Ringer′s solution. Three times gentle pressure was applied to the patch pipette, increasing the probability of mechanosensitive channels to be in the open state. Spontaneously activated channels are open briefly. (D) Tissue-specific gene expression analysis from adult zebrafish keratinocytes of two ion channel families containing mechanosensitive Ca2+-permeable channels. Genes found at multiple loci were amplified at consensus regions, with the exception of trpc4 and trpc5 that were analyzed separately. The chromosome number is given for genes that were found at multiple loci. Loading control is β-actin.