ZFIN ID: ZDB-FIG-131217-1
Graham et al., 2013 - Epidermal keratinocyte polarity and motility require Ca2+ influx through TRPV1. Journal of Cell Science   126(Pt 20):4602-13 Full text @ J. Cell Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Adult

Fig. 4

Ca2+ influx occurs predominantly through mechanosensitive channels. (A) xy-trajectory plot of cells exposed to 10µM Gd3+ for 1hour. Scale bar: 200µm. (B) Graph from transient shear stress applied to keratinocyte sheets indicates a transient increase in whole-cell [Ca2+]i during application of fluid flow (n = 3 cell sheets). (C) Whole-cell voltage clamp recording of a migrating keratinocyte in fish Ringer′s solution. Three times gentle pressure was applied to the patch pipette, increasing the probability of mechanosensitive channels to be in the open state. Spontaneously activated channels are open briefly. (D) Tissue-specific gene expression analysis from adult zebrafish keratinocytes of two ion channel families containing mechanosensitive Ca2+-permeable channels. Genes found at multiple loci were amplified at consensus regions, with the exception of trpc4 and trpc5 that were analyzed separately. The chromosome number is given for genes that were found at multiple loci. Loading control is β-actin.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
asic1a WT standard conditions Adult whole organism RTPCR
asic1b WT standard conditions Adult integument RTPCR
Adult whole organism RTPCR
asic1c WT standard conditions Adult whole organism RTPCR
asic2 WT standard conditions Adult whole organism RTPCR
asic4a WT standard conditions Adult whole organism RTPCR
asic4b WT standard conditions Adult whole organism RTPCR
erbb4a WT standard conditions Adult whole organism RTPCR
grhl1 WT standard conditions Adult integument RTPCR
Adult whole organism RTPCR
kita WT standard conditions Adult whole organism RTPCR
krt8 WT standard conditions Adult integument RTPCR
Adult whole organism RTPCR
pkd2 WT standard conditions Adult integument RTPCR
Adult keratinocyte RTPCR
Adult whole organism RTPCR
tp63 WT standard conditions Adult integument RTPCR
Adult whole organism RTPCR
trpa1a WT standard conditions Adult integument RTPCR
Adult keratinocyte RTPCR
Adult whole organism RTPCR
trpa1b WT standard conditions Adult whole organism RTPCR
trpc1 WT standard conditions Adult integument RTPCR
Adult whole organism RTPCR
trpc2b WT standard conditions Adult whole organism RTPCR
trpc4a WT standard conditions Adult whole organism RTPCR
trpc4b WT standard conditions Adult integument RTPCR
Adult whole organism RTPCR
trpc5a WT standard conditions Adult integument RTPCR
Adult whole organism RTPCR
trpc5b WT standard conditions Adult integument RTPCR
Adult whole organism RTPCR
trpc6a WT standard conditions Adult integument RTPCR
Adult keratinocyte RTPCR
Adult whole organism RTPCR
trpm1a WT standard conditions Adult whole organism RTPCR
trpm2 WT standard conditions Adult integument RTPCR
Adult keratinocyte RTPCR
Adult whole organism RTPCR
trpm3 WT standard conditions Adult integument RTPCR
Adult whole organism RTPCR
trpm4a WT standard conditions Adult integument RTPCR
Adult keratinocyte RTPCR
Adult whole organism RTPCR
trpm5 WT standard conditions Adult integument RTPCR
Adult keratinocyte RTPCR
Adult whole organism RTPCR
trpm7 WT standard conditions Adult integument RTPCR
Adult keratinocyte RTPCR
Adult whole organism RTPCR
trpn1 WT standard conditions Adult integument RTPCR
Adult whole organism RTPCR
trpv1 WT standard conditions Adult integument RTPCR
Adult keratinocyte RTPCR
Adult whole organism RTPCR
trpv4 WT standard conditions Adult integument RTPCR
Adult keratinocyte RTPCR
Adult whole organism RTPCR
trpv6 WT standard conditions Adult integument RTPCR
Adult keratinocyte RTPCR
Adult whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Journal of Cell Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Cell Sci.