ZFIN ID: ZDB-FIG-100525-32
Okuda et al., 2010 - B1 SOX coordinate cell specification with patterning and morphogenesis in the early zebrafish embryo. PLoS Genetics   6:e1000936 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 75%-epiboly to 10-13 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 1-4 somites to 10-13 somites

Fig. 3 Defects in embryo patterning, early neural development, and C&E movements in the QKD embryos.

Comparison of the gene expression profiles between uninjected control (Ctr) and QKD embryos. (A) Ventral expansion of early neural gene expression (otx2 [a], zic2b [b] and sox2/3/19a/19b [e–h]), and reduced expression of non-neural ectoderm marker genes (foxi1 [c] and dlx3b [d]) at 75%E. (B) Altered expression patterns of neural genes at the tailbud and 3-somite stages. The expression domains of gbx1 (c–e), eng2a (f) and hoxb1b (g, h) were anterolaterally shifted, which encompass the otx2 expression domain (a, b). (C) Defective neural development revealed by the loss of expression of neural marker genes, hesx1 (a), zic1 (b), rx3 (c), nkx1.2la (d) and krox20 (e). Residual expression of krox20 was observed in r5-derived neural crest cells. (D) Wider mesoderm structures with a shortened axis revealed by mesodermal marker genes, ntl (a) and myod1 (b). The stages and embryo orientations are shown in each panel: E, epiboly stage; S, somite stage; L, lateral view with dorsal to the right; D, dorsal view with anterior to the top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx3b WT control 75%-epiboly non neural ectoderm ISH
WT + MO1-sox19a + MO1-sox19b + MO2-sox19a + MO2-sox19b + MO3-sox2 + MO3-sox3 + MO4-sox2 + MO4-sox3 standard conditions 75%-epiboly non neural ectoderm ISH
egr2b WT control 10-13 somites rhombomere 3 ISH
10-13 somites rhombomere 5 ISH
WT + MO1-sox19a + MO1-sox19b + MO2-sox19a + MO2-sox19b + MO3-sox2 + MO3-sox3 + MO4-sox2 + MO4-sox3 standard conditions 10-13 somites Not Detected rhombomere 3 ISH
10-13 somites Not Detected rhombomere 5 ISH
en2a WT control 1-4 somites midbrain hindbrain boundary neural keel ISH
WT + MO1-sox19a + MO1-sox19b + MO2-sox19a + MO2-sox19b + MO3-sox2 + MO3-sox3 + MO4-sox2 + MO4-sox3 standard conditions 1-4 somites midbrain hindbrain boundary neural keel ISH
foxi1 WT control 75%-epiboly non neural ectoderm ISH
WT + MO1-sox19a + MO1-sox19b + MO2-sox19a + MO2-sox19b + MO3-sox2 + MO3-sox3 + MO4-sox2 + MO4-sox3 standard conditions 75%-epiboly non neural ectoderm ISH
gbx1 WT control Bud neural plate ISH
1-4 somites neural plate ISH
WT + MO1-sox19a + MO1-sox19b + MO2-sox19a + MO2-sox19b + MO3-sox2 + MO3-sox3 + MO4-sox2 + MO4-sox3 standard conditions 1-4 somites neural plate ISH
Bud neural plate ISH
hesx1 WT control Bud neural keel ISH
WT + MO1-sox19a + MO1-sox19b + MO2-sox19a + MO2-sox19b + MO3-sox2 + MO3-sox3 + MO4-sox2 + MO4-sox3 standard conditions Bud Not Detected neural keel ISH
hoxb1b WT control Bud neural plate ISH
WT + MO1-sox19a + MO1-sox19b + MO2-sox19a + MO2-sox19b + MO3-sox2 + MO3-sox3 + MO4-sox2 + MO4-sox3 standard conditions Bud neural plate ISH
myod1 WT control 10-13 somites mesoderm ISH
WT + MO1-sox19a + MO1-sox19b + MO2-sox19a + MO2-sox19b + MO3-sox2 + MO3-sox3 + MO4-sox2 + MO4-sox3 standard conditions 10-13 somites mesoderm ISH
nkx1.2la WT control 1-4 somites neural plate ISH
WT + MO1-sox19a + MO1-sox19b + MO2-sox19a + MO2-sox19b + MO3-sox2 + MO3-sox3 + MO4-sox2 + MO4-sox3 standard conditions 1-4 somites Not Detected neural plate ISH
otx2b WT control 75%-epiboly neuroectoderm ISH
Bud neural keel ISH
WT + MO1-sox19a + MO1-sox19b + MO2-sox19a + MO2-sox19b + MO3-sox2 + MO3-sox3 + MO4-sox2 + MO4-sox3 standard conditions 75%-epiboly neuroectoderm ISH
Bud neural keel ISH
rx3 WT control Bud neural plate ISH
WT + MO1-sox19a + MO1-sox19b + MO2-sox19a + MO2-sox19b + MO3-sox2 + MO3-sox3 + MO4-sox2 + MO4-sox3 standard conditions Bud Not Detected neural plate ISH
sox2 WT control 75%-epiboly neuroectoderm ISH
WT + MO1-sox19a + MO1-sox19b + MO2-sox19a + MO2-sox19b + MO3-sox2 + MO3-sox3 + MO4-sox2 + MO4-sox3 standard conditions 75%-epiboly neuroectoderm ISH
sox3 WT control 75%-epiboly neuroectoderm ISH
WT + MO1-sox19a + MO1-sox19b + MO2-sox19a + MO2-sox19b + MO3-sox2 + MO3-sox3 + MO4-sox2 + MO4-sox3 standard conditions 75%-epiboly neuroectoderm ISH
sox19a WT control 75%-epiboly neuroectoderm ISH
WT + MO1-sox19a + MO1-sox19b + MO2-sox19a + MO2-sox19b + MO3-sox2 + MO3-sox3 + MO4-sox2 + MO4-sox3 standard conditions 75%-epiboly neuroectoderm ISH
sox19b WT control 75%-epiboly neuroectoderm ISH
WT + MO1-sox19a + MO1-sox19b + MO2-sox19a + MO2-sox19b + MO3-sox2 + MO3-sox3 + MO4-sox2 + MO4-sox3 standard conditions 75%-epiboly neuroectoderm ISH
tbxta WT control Bud mesoderm ISH
WT + MO1-sox19a + MO1-sox19b + MO2-sox19a + MO2-sox19b + MO3-sox2 + MO3-sox3 + MO4-sox2 + MO4-sox3 standard conditions Bud mesoderm ISH
zic1 WT control Bud neural plate ISH
WT + MO1-sox19a + MO1-sox19b + MO2-sox19a + MO2-sox19b + MO3-sox2 + MO3-sox3 + MO4-sox2 + MO4-sox3 standard conditions Bud Not Detected neural plate ISH
zic2b WT control 75%-epiboly neuroectoderm ISH
WT + MO1-sox19a + MO1-sox19b + MO2-sox19a + MO2-sox19b + MO3-sox2 + MO3-sox3 + MO4-sox2 + MO4-sox3 standard conditions 75%-epiboly neuroectoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-sox19a + MO1-sox19b + MO2-sox19a + MO2-sox19b + MO3-sox2 + MO3-sox3 + MO4-sox2 + MO4-sox3 standard conditions Bud mesoderm increased width, abnormal
1-4 somites neural plate development disrupted, abnormal
Bud neural plate development disrupted, abnormal
10-13 somites mesoderm increased width, abnormal
10-13 somites neural plate development disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.