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ZFIN ID: ZDB-FIG-100525-31
Okuda et al., 2010 - B1 SOX coordinate cell specification with patterning and morphogenesis in the early zebrafish embryo. PLoS Genetics   6:e1000936 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 1-4 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Bud

Fig. 2 Rescue of the QKD phenotype by exogenous B1 sox mRNAs.

(A, B) Uninjected control (Ctr) embryos (A) and the QKD embryos (B). Live embryos were observed at 10–11 (a), 15–16 (b) and 30–31 (c) hpf. Expression of hesx1, pax2a and hoxb1b (d), dlx3b, hgg1 and ntl (e), and neurog1 (f) was visualized by whole-mount in situ hybridization. Lateral views (a–c); dorsal views with anterior to the top (d–f). (C) The QKD phenotype is similarly rescued by an exogenous supply of any B1 sox mRNA. The MOs for QKD were coinjected with the indicated mRNAs. In the B1 sox mRNA-coinjected embryos, the expression of hesx1, dlx3b and nuerog1 was recovered; patterning of the neural plate marked by pax2a and hoxb1b was normalized; and the expression patterns of hgg1, ntl and dlx3b reflecting C&E movements were also restored. Blue bracket, gap between the hgg1 and ntl expression domains; white dotted line, neural plate border. (D) Recovery of the AP axis elongation in QKD embryos injected with one of the B1 sox mRNAs. The length of the embryos at 15–16 hpf along the AP axis between the arrowheads (Ab, Bb, Cb) was measured for the uninjected control (Ctr) (n = 7), the QKD (n = 9) and QKD with B1 sox mRNA injection (sox2, n = 9; sox3, n = 6; sox19a, n = 7; sox19b, n = 6). The average AP axis lengths with standard errors are shown.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ctslb WT control Bud prechordal plate ISH
WT + MO1-sox19a + MO1-sox19b + MO2-sox19a + MO2-sox19b + MO3-sox2 + MO3-sox3 + MO4-sox2 + MO4-sox3 standard conditions Bud prechordal plate ISH
dlx3b WT control Bud neural plate ISH
WT + MO1-sox19a + MO1-sox19b + MO2-sox19a + MO2-sox19b + MO3-sox2 + MO3-sox3 + MO4-sox2 + MO4-sox3 standard conditions Bud Not Detected neural plate ISH
hesx1 WT control Bud neural keel ISH
WT + MO1-sox19a + MO1-sox19b + MO2-sox19a + MO2-sox19b + MO3-sox2 + MO3-sox3 + MO4-sox2 + MO4-sox3 standard conditions Bud Not Detected neural keel ISH
hoxb1b WT control Bud neural plate ISH
WT + MO1-sox19a + MO1-sox19b + MO2-sox19a + MO2-sox19b + MO3-sox2 + MO3-sox3 + MO4-sox2 + MO4-sox3 standard conditions Bud neural plate ISH
neurog1 WT control 1-4 somites neuron ISH
WT + MO1-sox19a + MO1-sox19b + MO2-sox19a + MO2-sox19b + MO3-sox2 + MO3-sox3 + MO4-sox2 + MO4-sox3 standard conditions 1-4 somites Not Detected neuron ISH
pax2a WT control Bud midbrain hindbrain boundary neural keel ISH
WT + MO1-sox19a + MO1-sox19b + MO2-sox19a + MO2-sox19b + MO3-sox2 + MO3-sox3 + MO4-sox2 + MO4-sox3 standard conditions Bud midbrain hindbrain boundary neural keel ISH
tbxta WT control Bud notochord ISH
WT + MO1-sox19a + MO1-sox19b + MO2-sox19a + MO2-sox19b + MO3-sox2 + MO3-sox3 + MO4-sox2 + MO4-sox3 standard conditions Bud notochord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-sox19a + MO1-sox19b + MO2-sox19a + MO2-sox19b + MO3-sox2 + MO3-sox3 + MO4-sox2 + MO4-sox3 standard conditions Bud axis shortened, abnormal
Bud central nervous system development disrupted, abnormal
Bud convergent extension disrupted, abnormal
Bud neural plate increased width, abnormal
Bud neurogenesis disrupted, abnormal
Bud notochord broad, abnormal
Bud prechordal plate increased distance notochord, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.