ZFIN ID: ZDB-FIG-201111-24
Kim et al., 2019 - Knockout of longevity gene Sirt1 in zebrafish leads to oxidative injury, chronic inflammation, and reduced life span. PLoS One   14:e0220581 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: Day 4 to Adult
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 4 to Adult

Fig. 3 Quantitative RT-PCR and western blot analysis.

Samples were prepared from (A, C) 4-dpf whole embryos, and (B, D) internal organs from 3-month-old zebrafish. Data are means, and bars indicate standard error. (A, B) Quantitative RT-PCR showed higher upregulation of inflammation-related genes in Sirt1-/- than in controls. (C, D) Western blot hybridization showed upregulation of IL-1b, active-caspase 3 and TGF-β. Control, wildtype; IL-1b, 18kDa; caspase 3a, 29 and 31 kDa; TGF-β, 45 kDa; β–actin, 43 kDa. *P < 0.05 versus control.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
casp3a AB standard conditions Day 4 whole organism WB
Adult whole organism WB
sirt1yu28/yu28(AB) standard conditions Day 4 whole organism WB
Adult whole organism WB
ifng1r AB standard conditions Day 4 whole organism RTPCR
Adult whole organism RTPCR
sirt1yu28/yu28(AB) standard conditions Day 4 whole organism RTPCR
Adult whole organism RTPCR
il1b AB standard conditions Day 4 whole organism RTPCR
Day 4 whole organism WB
Adult whole organism RTPCR
Adult whole organism WB
sirt1yu28/yu28(AB) standard conditions Day 4 whole organism RTPCR
Day 4 whole organism WB
Adult whole organism RTPCR
Adult whole organism WB
il6 AB standard conditions Day 4 whole organism RTPCR
Adult whole organism RTPCR
sirt1yu28/yu28(AB) standard conditions Day 4 whole organism RTPCR
Adult whole organism RTPCR
mmp2 AB standard conditions Day 4 whole organism RTPCR
sirt1yu28/yu28(AB) standard conditions Day 4 whole organism RTPCR
mmp9 AB standard conditions Day 4 whole organism RTPCR
sirt1yu28/yu28(AB) standard conditions Day 4 whole organism RTPCR
nfe2l2a AB standard conditions Day 4 whole organism RTPCR
sirt1yu28/yu28(AB) standard conditions Day 4 whole organism RTPCR
nos2a AB standard conditions Day 4 whole organism RTPCR
Adult whole organism RTPCR
sirt1yu28/yu28(AB) standard conditions Day 4 whole organism RTPCR
Adult whole organism RTPCR
ppargc1a AB standard conditions Day 4 whole organism RTPCR
sirt1yu28/yu28(AB) standard conditions Day 4 whole organism RTPCR
ptgs2a AB standard conditions Day 4 whole organism RTPCR
sirt1yu28/yu28(AB) standard conditions Day 4 whole organism RTPCR
sirt1 AB standard conditions Day 4 whole organism RTPCR
sirt1yu28/yu28(AB) standard conditions Day 4 whole organism RTPCR
socs1a AB standard conditions Day 4 whole organism RTPCR
Adult whole organism RTPCR
sirt1yu28/yu28(AB) standard conditions Day 4 whole organism RTPCR
Adult whole organism RTPCR
socs3a AB standard conditions Day 4 whole organism RTPCR
Adult whole organism RTPCR
sirt1yu28/yu28(AB) standard conditions Day 4 whole organism RTPCR
Adult whole organism RTPCR
sod1 AB standard conditions Day 4 whole organism RTPCR
sirt1yu28/yu28(AB) standard conditions Day 4 whole organism RTPCR
tgfb1a AB standard conditions Day 4 whole organism WB
Day 4 whole organism RTPCR
Adult whole organism WB
Adult whole organism RTPCR
sirt1yu28/yu28(AB) standard conditions Day 4 whole organism RTPCR
Day 4 whole organism WB
Adult whole organism RTPCR
Adult whole organism WB
tgfb2 AB standard conditions Day 4 whole organism RTPCR
Adult whole organism RTPCR
sirt1yu28/yu28(AB) standard conditions Day 4 whole organism RTPCR
Adult whole organism RTPCR
timp2a AB standard conditions Day 4 whole organism RTPCR
Adult whole organism RTPCR
sirt1yu28/yu28(AB) standard conditions Day 4 whole organism RTPCR
Adult whole organism RTPCR
tnfa AB standard conditions Day 4 whole organism RTPCR
Adult whole organism RTPCR
sirt1yu28/yu28(AB) standard conditions Day 4 whole organism RTPCR
Adult whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sirt1yu28/yu28(AB) standard conditions Day 4 inflammatory response increased process quality, abnormal
Day 4 whole organism casp3a expression increased amount, abnormal
Day 4 whole organism il1b expression increased amount, abnormal
Day 4 whole organism il1b expression increased amount, abnormal
Day 4 whole organism il6 expression increased amount, abnormal
Day 4 whole organism nfe2l2a expression increased amount, abnormal
Day 4 whole organism nos2a expression increased amount, abnormal
Day 4 whole organism socs1a expression increased amount, abnormal
Day 4 whole organism socs3a expression increased amount, abnormal
Day 4 whole organism tgfb1a expression increased amount, abnormal
Day 4 whole organism tgfb1a expression increased amount, abnormal
Adult inflammatory response increased process quality, abnormal
Adult whole organism casp3a expression increased amount, abnormal
Adult whole organism il1b expression increased amount, abnormal
Adult whole organism il1b expression increased amount, abnormal
Adult whole organism il6 expression increased amount, abnormal
Adult whole organism socs1a expression increased amount, abnormal
Adult whole organism tgfb1a expression increased amount, abnormal
Adult whole organism tnfa expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One