FIGURE

Fig. 1

ID

ZDB-FIG-201111-23

Publication

Kim et al., 2019 - Knockout of longevity gene Sirt1 in zebrafish leads to oxidative injury, chronic inflammation, and reduced life span

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Fig. 1

Sirt1 Knockout by Crispr/Cas9 technique.

(A) Schematic illustration of the Sirt1 locus. Exon 1 of the Sirt1 gene was targeted. The CRISPR target site and PAM motif are indicated. (B) Sequence confirmation of four-nucleotide insertion mutations. (C) Predicted truncation of the SIRT1 protein. (D) Expression of the Sirt1 mutant was verified by western blot using an antibody of Sirt1; ?-actin was used as a loading control.

Expression Data

Gene:	sirt1
Fish:	AB sirt1^yu28/yu28 (AB)
Anatomical Term:	whole organism
Stage:	Day 4

Expression Detail

Gene	Antibody	Fish	Conditions	Stage	Qualifier	Anatomy	Assay
		AB	standard conditions	Day 4		whole organism	WB
		sirt1^yu28/yu28 (AB)	standard conditions	Day 4	Not Detected	whole organism	WB

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Antibody Labeling

No data available

Phenotype Data

Fish:	sirt1^yu28/yu28 (AB)
Observed In:	whole organism
Stage:	Day 4

Phenotype Detail

Fish	Conditions	Stage	Phenotype
sirt1^yu28/yu28 (AB)	standard conditions	Day 4	whole organism sirt1 expression absent, abnormal

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Acknowledgments

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