header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-080715-22
Krens et al., 2008 - Distinct functions for ERK1 and ERK2 in cell migration processes during zebrafish gastrulation. Developmental Biology   319(2):370-383 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Shield to 90%-epiboly

Fig. S3 Expression of embryonic patterning marker genes in ERK1 and ERK2 morphants Expression of mRNA was detected by whole mount in situ hybridization. (A–F, J–L); animal pole view, dorsal to right, (G–I,M–O) dorsal view, animal pole to top, (P–G′) lateral view, dorsal to right, animal pole to top. (A–C, P–R) gata2, expressed in non-neural ectoderm, at 80% epiboly. (D–F, SU) otx2, expressed in ectoderm at, 90% epiboly. (G–I, V–X)) cyclops/ndr2 in axial mesoderm at 80–90% epiboly. (J–L) snail1a in paraxial mesoderm at 90% epiboly. (M–O) sox17 in endoderm and forerunner cells at 80% epiboly. (Y–A′) notail in margin an axial mesoderm at 90% epiboly. (B′–D′) chordin in presumptive dorsal shield. (E′–G′) goosecoid in presumptive dorsal shield. Arrowheads mark the different boundaries of the different marker gene expression domains; the dotted line in P–X marks the boundary of epiboly progression.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
chrd WT standard conditions Shield shield ISH
WT + MO1-mapk1 standard conditions Shield shield ISH
WT + MO3-mapk3 standard conditions Shield shield ISH
gata2a WT standard conditions 75%-epiboly non neural ectoderm ISH
WT + MO1-mapk1 standard conditions 75%-epiboly non neural ectoderm ISH
WT + MO3-mapk3 standard conditions 75%-epiboly non neural ectoderm ISH
gsc WT standard conditions Shield shield ISH
WT + MO1-mapk1 standard conditions Shield shield ISH
WT + MO3-mapk3 standard conditions Shield shield ISH
ndr2 WT standard conditions 90%-epiboly axial mesoderm ISH
WT + MO1-mapk1 standard conditions 90%-epiboly axial mesoderm ISH
WT + MO3-mapk3 standard conditions 90%-epiboly axial mesoderm ISH
otx2b WT standard conditions 90%-epiboly ectoderm ISH
WT + MO1-mapk1 standard conditions 90%-epiboly ectoderm ISH
WT + MO3-mapk3 standard conditions 90%-epiboly ectoderm ISH
snai1a WT standard conditions 90%-epiboly presumptive paraxial mesoderm ISH
WT + MO1-mapk1 standard conditions 90%-epiboly presumptive paraxial mesoderm ISH
WT + MO3-mapk3 standard conditions 90%-epiboly presumptive paraxial mesoderm ISH
sox17 WT standard conditions 75%-epiboly endoderm ISH
75%-epiboly forerunner cell group ISH
WT + MO1-mapk1 standard conditions 75%-epiboly endoderm ISH
75%-epiboly forerunner cell group ISH
WT + MO3-mapk3 standard conditions 75%-epiboly endoderm ISH
75%-epiboly forerunner cell group ISH
tbxta WT standard conditions 90%-epiboly axial mesoderm ISH
90%-epiboly margin ISH
WT + MO1-mapk1 standard conditions 90%-epiboly axial mesoderm ISH
90%-epiboly margin ISH
WT + MO3-mapk3 standard conditions 90%-epiboly axial mesoderm ISH
90%-epiboly margin ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 319(2), Krens, S.F., He, S., Lamers, G.E., Meijer, A.H., Bakkers, J., Schmidt, T., Spaink, H.P., and Snaar-Jagalska, B.E., Distinct functions for ERK1 and ERK2 in cell migration processes during zebrafish gastrulation, 370-383, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.