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ZFIN ID: ZDB-FIG-080715-13
Krens et al., 2008 - Distinct functions for ERK1 and ERK2 in cell migration processes during zebrafish gastrulation. Developmental Biology   319(2):370-383 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Bud
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Bud

Fig. 2 Phenotypic characterization of ERK1 and ERK2 morphants in late-gastrulation and early segmentation indicates affected cell migration in CE movements. (A, D, G, J); wild type control embryos, (B, E, H, K); ERK1 knockdown embryos, injected with 0.2 mM ERK1MO, (C, F, I, L); ERK2 knockdown embryos, injected with 0.2 mM ERK2MO. (A–C); Live embryos at yolk plug closure (YPC) to tailbud (TB) stages, animal pole is up, dorsal to right in wild type, ERK1MO or ERK1MO injected embryos, The distance between the arrowheads resembles the length of the AP body axis (Zeiss EC Plan-Neofluar 10x/0.30 objective), or dorsal view (D–F), white spacer highlights the widening of the dorsal notochord (Zeiss Plan-Neofluar 40x/0.75 ∞/0.17 objective). (G–L); Combined in situ hybridization on 10-hpf old embryos with dlx3 (edge neural plate) with shh (midline) or with hgg1 (hatching gland) marker genes (anterior view, dorsal to top).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctslb WT standard conditions Bud polster ISH
WT + MO1-mapk1 standard conditions Bud polster ISH
WT + MO3-mapk3 standard conditions Bud polster ISH
dlx3b WT standard conditions Bud neural plate ISH
WT + MO1-mapk1 standard conditions Bud neural plate ISH
WT + MO3-mapk3 standard conditions Bud neural plate ISH
shha WT standard conditions Bud axis ISH
WT + MO1-mapk1 standard conditions Bud axis ISH
WT + MO3-mapk3 standard conditions Bud axis ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-mapk1 standard conditions Bud notochord increased width, abnormal
Bud whole organism decreased length, abnormal
WT + MO3-mapk3 standard conditions Bud notochord increased width, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 319(2), Krens, S.F., He, S., Lamers, G.E., Meijer, A.H., Bakkers, J., Schmidt, T., Spaink, H.P., and Snaar-Jagalska, B.E., Distinct functions for ERK1 and ERK2 in cell migration processes during zebrafish gastrulation, 370-383, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.