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ZFIN ID: ZDB-FIG-080715-12
Krens et al., 2008 - Distinct functions for ERK1 and ERK2 in cell migration processes during zebrafish gastrulation. Developmental Biology   319(2):370-383 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: 20-25 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 1 Specific ERK1 and ERK2 knockdown by morpholino injection. (A–C) Images show representative examples of the ERK1 (A) or ERK2 (B) knockdown phenotypes at 24 hpf, compared to wild type (wt) embryos (C). (D) Specific knockdown was confirmed by Western blot analysis optimized to discriminate between the sizes of ERK1 (p44MAPK) and ERK2 (p42MAPK) protein and detected with global ERK antibody (D). Protein samples were isolated from 20 hpf embryos, injected with 0.2 mM ERK1MO or ERK1MO. (E) Statistics of ERK knockdown and (cross-) rescue experiments determined by co-injection of ERK1 or ERK2 morpholino with synthetic erk1 or erk2 mRNA at 24 hpf. Black = wild type; gray = phenotype; white = dead. (F–M) Images show phenotypes of surviving knockdown embryos at 48 hpf (F, G), embryos rescued by co-injection of corresponding mRNA (8pg erk1 RNA, 1.5pg erk2 RNA) (H, I), embryos injected with mRNA only (J, K) and wild type embryos at 48 hpf (L, M).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mapk1 WT + MO1-mapk1 standard conditions 20-25 somites whole organism WB
mapk3 WT + MO1-mapk1 standard conditions 20-25 somites whole organism WB
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-mapk1 standard conditions Prim-5 whole organism decreased length, abnormal
WT + MO1-mapk1 standard conditions Prim-5 whole organism deformed, abnormal
WT + MO1-mapk1 standard conditions Long-pec pericardial cavity edematous, abnormal
WT + MO1-mapk1 standard conditions Long-pec whole organism decreased length, abnormal
WT + MO3-mapk3 standard conditions Prim-5 somite deformed, abnormal
Prim-5 whole organism decreased length, abnormal
Long-pec pericardial cavity edematous, abnormal
Long-pec whole organism decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 319(2), Krens, S.F., He, S., Lamers, G.E., Meijer, A.H., Bakkers, J., Schmidt, T., Spaink, H.P., and Snaar-Jagalska, B.E., Distinct functions for ERK1 and ERK2 in cell migration processes during zebrafish gastrulation, 370-383, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.