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ZFIN ID: ZDB-FIG-080715-17
Krens et al., 2008 - Distinct functions for ERK1 and ERK2 in cell migration processes during zebrafish gastrulation. Developmental Biology   319(2):370-383 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Dome

Fig. 6 Saturating knockdown of ERK2 prohibits epiboly initiation and revealed ERK2 to be the active MAPK in the margin. Embryos were injected with 0.4 mM ERK1MO (B, E, H, K, N, Q) or ERK2MO (C, F, I, L, O, R) and compared to wild type embryos (A, D, G, J, M, P). Nomarski/differential interference contrast (DIC) microscopy using a Zeiss EC Plan-Neofluar 10x/0.30 objective (A, B, C) and a Zeiss Plan-Neofluar 40x/0.75 ∞/0.17 (D, E, F) was used to monitor the margin at the onset of gastrulation (4.5 hpf). Localization of dpERK was detected by immuno-localization in wild type, ERK1MO and ERK2MO injected embryos at 4.5 hpf (G–I) and 8 hpf (J–L) by phospho-specific ERK antibody. Images in panels G–L were taken by confocal laser scanning microscopy. Mesoderm formation was followed using in situ hybridization markers ntl (M–O) and tbx6 (P–R), top view, presumptive dorsal side right.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
tbx16l WT standard conditions Dome presumptive mesoderm ISH
WT + MO1-mapk1 standard conditions Dome presumptive mesoderm ISH
WT + MO3-mapk3 standard conditions Dome presumptive mesoderm ISH
tbxta WT standard conditions Dome presumptive mesoderm ISH
WT + MO1-mapk1 standard conditions Dome presumptive mesoderm ISH
WT + MO3-mapk3 standard conditions Dome presumptive mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 319(2), Krens, S.F., He, S., Lamers, G.E., Meijer, A.H., Bakkers, J., Schmidt, T., Spaink, H.P., and Snaar-Jagalska, B.E., Distinct functions for ERK1 and ERK2 in cell migration processes during zebrafish gastrulation, 370-383, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.