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ZFIN ID: ZDB-FIG-161018-22
Olsen et al., 2016 - G9a and ZNF644 Physically Associate to Suppress Progenitor Gene Expression during Neurogenesis. Stem Cell Reports   7(3):454-70 Full text @ Stem Cell Reports
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 3

znf644a and znf644b Are Required for H3K9me2-Mediated Gene Silencing in the Forming Retina

(A) Frequency and distance from TSS at which H3K9me2 peaks were identified in WT, znf644a, and znf644b morphants.

(B) Venn diagram illustrating the number and overlap of H3K9me2 peaks at 48 hpf in znf644a or znf644b morphants or WT embryos.

(C) ChIP-PCR assays monitoring H3K9me2 levels near the TSSs of vsx2 or ccnd1 genes at 48 hpf.

(D) WISH assays monitoring expression of progenitor or proneural genes in WT, znf644a, or znf644b morphant retinas. Arrows highlight expression domains.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
atoh7 AB standard conditions Long-pec retina neuroblast ISH
AB + MO1-znf644a standard conditions Long-pec retina neuroblast ISH
AB + MO1-znf644b standard conditions Long-pec retina neuroblast ISH
ccnd1 AB standard conditions Long-pec retina ISH
AB + MO1-znf644a standard conditions Long-pec retina central region ISH
AB + MO1-znf644b standard conditions Long-pec retina central region ISH
otx2b AB standard conditions Protruding-mouth retina multi fate stem cell ISH
AB + MO1-znf644a standard conditions Protruding-mouth retina multi fate stem cell ISH
AB + MO1-znf644b standard conditions Protruding-mouth retina multi fate stem cell ISH
rx3 AB standard conditions Long-pec retina multi fate stem cell ISH
AB + MO1-znf644a standard conditions Long-pec retina multi fate stem cell ISH
AB + MO1-znf644b standard conditions Long-pec retina multi fate stem cell ISH
vsx2 AB standard conditions Long-pec retina ISH
AB + MO1-znf644a standard conditions Long-pec retina central region ISH
AB + MO1-znf644b standard conditions Long-pec retina central region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-znf644a standard conditions Long-pec regulation of gene silencing disrupted, abnormal
Long-pec retina central region vsx2 expression mislocalised, abnormal
Long-pec retina central region ccnd1 expression mislocalised, abnormal
Long-pec retina central region ccnd1 expression spatial pattern, abnormal
Long-pec retina central region vsx2 expression spatial pattern, abnormal
Long-pec retina multi fate stem cell rx3 expression increased amount, abnormal
Long-pec retina multi fate stem cell rx3 expression spatial pattern, abnormal
Long-pec retina neuroblast atoh7 expression increased amount, abnormal
Long-pec retina neuroblast atoh7 expression spatial pattern, abnormal
Protruding-mouth retina multi fate stem cell otx2b expression increased amount, abnormal
Protruding-mouth retina multi fate stem cell otx2b expression spatial pattern, abnormal
AB + MO1-znf644b standard conditions Long-pec regulation of gene silencing disrupted, abnormal
Long-pec retina central region vsx2 expression mislocalised, abnormal
Long-pec retina central region ccnd1 expression mislocalised, abnormal
Long-pec retina central region vsx2 expression spatial pattern, abnormal
Long-pec retina central region ccnd1 expression spatial pattern, abnormal
Protruding-mouth retina multi fate stem cell otx2b expression increased amount, abnormal
Protruding-mouth retina multi fate stem cell otx2b expression spatial pattern, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Stem Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Stem Cell Reports