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ZFIN ID: ZDB-FIG-161018-32
Olsen et al., 2016 - G9a and ZNF644 Physically Associate to Suppress Progenitor Gene Expression during Neurogenesis. Stem Cell Reports   7(3):454-70 Full text @ Stem Cell Reports
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. S6

Interaction of g9a and znf644 genes in regulating progenitor cell cycle. (A) WISH assays monitoring the expression of ccnd1 in (top) midbrain or (bottom) retinal cross-sections at 48 hpf from WT embryos or embryos injected with the indicated combined subthreshold MO doses. (B) Immunostaining assays monitoring pH3 levels or BrdU incorporation at 48 hpf from WT or embryos injected with the indicated combined subthreshold MO doses. (C) Rescue experiments in which mRNA encoding WT human (ZNF644) or mutant version lacking G9a/GLP binding capability (C1263A) is coinjected with znf644a-MO (left) or znf644b-MO (right). Vsx2 expression was assessed by WISH at 48 hpf, and PCNA, pH3, and Zpr1 levels (n=3 in each group) were assessed by immunostaining and confocal microscopy at the indicated time points.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ccnd1 AB standard conditions Long-pec retina ISH
AB + MO1-ehmt2 + MO1-znf644a standard conditions Long-pec midbrain ISH
Long-pec retina central region ISH
AB + MO1-ehmt2 + MO1-znf644b standard conditions Long-pec midbrain ISH
Long-pec retina central region ISH
AB + MO1-znf644a + MO1-znf644b standard conditions Long-pec midbrain ISH
Long-pec retina central region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-ehmt2 + MO1-znf644a standard conditions Long-pec midbrain ccnd1 expression mislocalised, abnormal
AB + MO1-ehmt2 + MO1-znf644a standard conditions Long-pec midbrain ccnd1 expression spatial pattern, abnormal
AB + MO1-ehmt2 + MO1-znf644a standard conditions Long-pec retina cell population proliferation increased occurrence, abnormal
AB + MO1-ehmt2 + MO1-znf644a standard conditions Long-pec retina central region ccnd1 expression mislocalised, abnormal
AB + MO1-ehmt2 + MO1-znf644a standard conditions Long-pec retina central region ccnd1 expression spatial pattern, abnormal
AB + MO1-ehmt2 + MO1-znf644b standard conditions Long-pec midbrain ccnd1 expression mislocalised, abnormal
Long-pec midbrain ccnd1 expression spatial pattern, abnormal
Long-pec retina cell population proliferation increased occurrence, abnormal
Long-pec retina central region ccnd1 expression mislocalised, abnormal
Long-pec retina central region ccnd1 expression spatial pattern, abnormal
AB + MO1-znf644a + MO1-znf644b standard conditions Long-pec midbrain ccnd1 expression mislocalised, abnormal
Long-pec midbrain ccnd1 expression spatial pattern, abnormal
Long-pec retina cell population proliferation increased occurrence, abnormal
Long-pec retina central region ccnd1 expression mislocalised, abnormal
Long-pec retina central region ccnd1 expression spatial pattern, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Stem Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Stem Cell Reports