ZFIN ID: ZDB-FIG-161018-26
Olsen et al., 2016 - G9a and ZNF644 Physically Associate to Suppress Progenitor Gene Expression during Neurogenesis. Stem Cell Reports   7(3):454-70 Full text @ Stem Cell Reports
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 7

The Retinal Defects of g9a, znf644a, and znf644b Morphants Are Recapitulated in the Midbrain

(A) WISH assays monitoring ccnd1 expression in WT, znf644a, or znf644b morphant midbrain cross-sections at 48 hpf. Arrows indicate mislocalized ccnd1 expression.

(B) Immunostaining of cleaved Caspase3 in g9a or znf644b morphant midbrain cross-sections at 72 hpf and rescue by p53-MO (n = 3 in each group). Arrows denote the position of Caspase3+ cells.

(C) Immunostaining of PCNA in WT or znf644a morphant midbrain cross-sections (n = 3 in each group). Arrows denote the position of PCNA+ cells.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ccnd1 AB standard conditions Long-pec midbrain ISH
AB + MO1-znf644a standard conditions Long-pec midbrain ISH
AB + MO1-znf644b standard conditions Long-pec midbrain ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-ehmt2 standard conditions Protruding-mouth midbrain apoptotic process increased occurrence, abnormal
AB + MO1-znf644a standard conditions Long-pec cell proliferation in midbrain increased occurrence, abnormal
Long-pec midbrain ccnd1 expression mislocalised, abnormal
Long-pec midbrain ccnd1 expression spatial pattern, abnormal
AB + MO1-znf644b standard conditions Long-pec midbrain ccnd1 expression mislocalised, abnormal
Long-pec midbrain ccnd1 expression spatial pattern, abnormal
Protruding-mouth midbrain apoptotic process increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Stem Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Stem Cell Reports