ZFIN ID: ZDB-FIG-161018-26
Olsen et al., 2016 - G9a and ZNF644 Physically Associate to Suppress Progenitor Gene Expression during Neurogenesis. Stem Cell Reports   7(3):454-70 Full text @ Stem Cell Reports
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 7

The Retinal Defects of g9a, znf644a, and znf644b Morphants Are Recapitulated in the Midbrain

(A) WISH assays monitoring ccnd1 expression in WT, znf644a, or znf644b morphant midbrain cross-sections at 48 hpf. Arrows indicate mislocalized ccnd1 expression.

(B) Immunostaining of cleaved Caspase3 in g9a or znf644b morphant midbrain cross-sections at 72 hpf and rescue by p53-MO (n = 3 in each group). Arrows denote the position of Caspase3+ cells.

(C) Immunostaining of PCNA in WT or znf644a morphant midbrain cross-sections (n = 3 in each group). Arrows denote the position of PCNA+ cells.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ccnd1 AB standard conditions Long-pec midbrain ISH
AB + MO1-znf644a standard conditions Long-pec midbrain ISH
AB + MO1-znf644b standard conditions Long-pec midbrain ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-ehmt2 standard conditions Protruding-mouth midbrain apoptotic process increased occurrence, abnormal
AB + MO1-znf644a standard conditions Long-pec cell proliferation in midbrain increased occurrence, abnormal
Long-pec midbrain ccnd1 expression mislocalised, abnormal
Long-pec midbrain ccnd1 expression spatial pattern, abnormal
AB + MO1-znf644b standard conditions Long-pec midbrain ccnd1 expression mislocalised, abnormal
Long-pec midbrain ccnd1 expression spatial pattern, abnormal
Protruding-mouth midbrain apoptotic process increased occurrence, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Stem Cell Reports