Fig. 4

Olsen et al., 2016 - G9a and ZNF644 Physically Associate to Suppress Progenitor Gene Expression during Neurogenesis
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Fig. 4

znf644a and znf644b Morphant Retinas Are Composed of Distinct Populations of Retinal Cells with Differing Characteristics

(A) Immunostaining of retinal cross-sections monitoring BrdU incorporation and PCNA+ cells at 48 hpf in WT, znf644a, and znf644b morphants.

(B) Quantitation of BrdU+ cells/¼m2 in central retina of WT, znf644a, or znf644b morphants at 48 hpf (n = 3 for each group). Error bars represent SD. p < 0.05, Student′s t test.

(C–E) Immunostaining of pH3 expression in retinal cross-sections at 48 and 72 hpf in WT, and znf644a morphants (C). Quantitation of pH3+ cells/¼m2 in the central retina of WT, znf644a, or znf644b morphants at (D) 48 hpf (n = 5 for each group) and (E) 72 hpf (n = 2 for each group). Quantitation represented as mean ± SD.

(F) Immunostaining monitoring marker protein expression in retinal cross-sections. At 72 hpf, amacrine cells express GABA, bipolar neurons express PKC, and cone photoreceptors express Zpr1.

White arrows highlight populations of proliferative or differentiated cells.

Expression Data
Knockdown Reagents:
Anatomical Terms:
Stage: Protruding-mouth

Expression Detail
Antibody Labeling
Phenotype Data
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Phenotype Detail
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