ZFIN ID: ZDB-FIG-161018-23
Olsen et al., 2016 - G9a and ZNF644 Physically Associate to Suppress Progenitor Gene Expression during Neurogenesis. Stem Cell Reports   7(3):454-70 Full text @ Stem Cell Reports
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 4

znf644a and znf644b Morphant Retinas Are Composed of Distinct Populations of Retinal Cells with Differing Characteristics

(A) Immunostaining of retinal cross-sections monitoring BrdU incorporation and PCNA+ cells at 48 hpf in WT, znf644a, and znf644b morphants.

(B) Quantitation of BrdU+ cells/¼m2 in central retina of WT, znf644a, or znf644b morphants at 48 hpf (n = 3 for each group). Error bars represent SD. p < 0.05, Student′s t test.

(C–E) Immunostaining of pH3 expression in retinal cross-sections at 48 and 72 hpf in WT, and znf644a morphants (C). Quantitation of pH3+ cells/¼m2 in the central retina of WT, znf644a, or znf644b morphants at (D) 48 hpf (n = 5 for each group) and (E) 72 hpf (n = 2 for each group). Quantitation represented as mean ± SD.

(F) Immunostaining monitoring marker protein expression in retinal cross-sections. At 72 hpf, amacrine cells express GABA, bipolar neurons express PKC, and cone photoreceptors express Zpr1.

White arrows highlight populations of proliferative or differentiated cells.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-GABA IHC AB standard conditions Protruding-mouth amacrine cell
IHC AB + MO1-znf644a standard conditions Protruding-mouth Not Detected amacrine cell
IHC AB + MO1-znf644b standard conditions Protruding-mouth amacrine cell
Ab2-prkcb IHC AB standard conditions Protruding-mouth retinal bipolar neuron
IHC AB + MO1-znf644a standard conditions Protruding-mouth Not Detected retinal bipolar neuron
IHC AB + MO1-znf644b standard conditions Protruding-mouth retinal bipolar neuron
zpr-1 IHC AB standard conditions Protruding-mouth retinal cone cell
IHC AB + MO1-znf644a standard conditions Protruding-mouth Not Detected retinal cone cell
IHC AB + MO1-znf644b standard conditions Protruding-mouth retinal cone cell
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-znf644a standard conditions Long-pec retina cell population proliferation increased occurrence, abnormal
Protruding-mouth amacrine cell ab1-gaba labeling absent, abnormal
Protruding-mouth retina cell population proliferation increased occurrence, abnormal
Protruding-mouth retinal bipolar neuron ab2-prkcb labeling absent, abnormal
Protruding-mouth retinal cone cell zpr-1 labeling absent, abnormal
AB + MO1-znf644b standard conditions Long-pec retina cell population proliferation increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Stem Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Stem Cell Reports