ZFIN ID: ZDB-FIG-161018-21
Olsen et al., 2016 - G9a and ZNF644 Physically Associate to Suppress Progenitor Gene Expression during Neurogenesis. Stem Cell Reports   7(3):454-70 Full text @ Stem Cell Reports
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 2

Zebrafish znf644 Paralogs Are Specifically Expressed In and Regulate the Development of Retinal and Midbrain Progenitor Cells

(A) Conservation of C2H2-like ZF motifs between human and zebrafish ZNF644 genes.

(B) Representative lateral views, retinal cross-sections, and midbrain cross-sections of whole-mount in situ hybridization (WISH) assays monitoring znf644a or znf644b expression at 24 hpf (n > 15 in each group). Arrows denote the inner part of the central retinal epithelium.

(C) Lateral views of g9a (20/32), znf644a (10/18), or znf644b (14/21) morphant embryos compared with WT (21/21). Dashed circles denote 2D area of the WT retina. Arrowheads denote the dorsal hindbrain region.

(D) Quantitation (mean ± SD) of retinal 2D cross-sectional area (¼m2) of znf644a, znf644b, or g9a morphants relative to WT embryos. Error bars represent SD. *p < 0.005, Student′s t test.

(E) RT-PCR assays monitoring unspliced (unspl) and spliced (spl) znf644a or znf644b transcripts at 24 hpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
znf644a AB standard conditions Prim-5 midbrain ISH
Prim-5 presumptive retinal pigmented epithelium ISH
znf644b AB standard conditions Prim-5 midbrain ISH
Prim-5 presumptive retinal pigmented epithelium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-ehmt2 standard conditions Prim-5 eye decreased size, abnormal
Prim-5 midbrain morphology, abnormal
AB + MO1-znf644a standard conditions Prim-5 eye decreased size, abnormal
Prim-5 midbrain morphology, abnormal
AB + MO1-znf644b standard conditions Prim-5 eye decreased size, abnormal
Prim-5 midbrain morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Stem Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Stem Cell Reports