ZFIN ID: ZDB-FIG-161018-21
Olsen et al., 2016 - G9a and ZNF644 Physically Associate to Suppress Progenitor Gene Expression during Neurogenesis. Stem Cell Reports   7(3):454-70 Full text @ Stem Cell Reports
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 2

Zebrafish znf644 Paralogs Are Specifically Expressed In and Regulate the Development of Retinal and Midbrain Progenitor Cells

(A) Conservation of C2H2-like ZF motifs between human and zebrafish ZNF644 genes.

(B) Representative lateral views, retinal cross-sections, and midbrain cross-sections of whole-mount in situ hybridization (WISH) assays monitoring znf644a or znf644b expression at 24 hpf (n > 15 in each group). Arrows denote the inner part of the central retinal epithelium.

(C) Lateral views of g9a (20/32), znf644a (10/18), or znf644b (14/21) morphant embryos compared with WT (21/21). Dashed circles denote 2D area of the WT retina. Arrowheads denote the dorsal hindbrain region.

(D) Quantitation (mean ± SD) of retinal 2D cross-sectional area (¼m2) of znf644a, znf644b, or g9a morphants relative to WT embryos. Error bars represent SD. *p < 0.005, Student′s t test.

(E) RT-PCR assays monitoring unspliced (unspl) and spliced (spl) znf644a or znf644b transcripts at 24 hpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
znf644a AB standard conditions Prim-5 midbrain ISH
Prim-5 presumptive retinal pigmented epithelium ISH
znf644b AB standard conditions Prim-5 midbrain ISH
Prim-5 presumptive retinal pigmented epithelium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-ehmt2 standard conditions Prim-5 eye decreased size, abnormal
Prim-5 midbrain morphology, abnormal
AB + MO1-znf644a standard conditions Prim-5 eye decreased size, abnormal
Prim-5 midbrain morphology, abnormal
AB + MO1-znf644b standard conditions Prim-5 eye decreased size, abnormal
Prim-5 midbrain morphology, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Stem Cell Reports