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ZFIN ID: ZDB-FIG-130123-65
Bhat et al., 2013 - A gene network that coordinates preplacodal competence and neural crest specification in zebrafish. Developmental Biology   373(1):107-117 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 75%-epiboly to Prim-15
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Bud to Prim-15

Fig. 1 Knockdown of tfap2a/c impairs early placode development. A–G, Expression of early placodal markers in control embryos and tfap2a/c morphants. Expression of pitx3 at 12.5 hpf marks pituitary/olfactory/lens precursors (A); cxcr4b marks the olfactory pit at 30 hpf (B); foxe3 marks the lens at 30 hpf (C); neuroD marks the trigeminal (tg) and anterior lateral line (al) at 14 hpf (D); pax8 at 10 hpf (E) and pax2a at 12 hpf (F) mark otic/epibranchial precursors; phox2a marks epibranchial ganglia (eg) at 30 hpf (G). H–J, Expression of competence factors foxi1 (H) and gata3 (I), and PPE marker dlx3b (J), at 8.5 hpf in control embryos and tfap2a/c morphants. K and L, Expression of tfap2a (K) and tfap2c (L) at 8.5 hpf in control embryos and tfap2a-/- (lockjaw) mutants. M, otic vesicle at 30 hpf in a control embryo, tfap2a/c morphant, and embryos injected with sub-effective doses (0.5 ng) of foxi1-MO, dlx3b-MO or both foxi1-MO and dlx3b-MO. Sub-effective doses of these MO have little or no effect by themselves, but when combined have synergistic effects that phenocopy the tfap2a/c morphant. Images show dorsal views with anterior up (A, D–F), lateral views with anterior to the left (B, C, G, M) or lateral views with dorsal to the right (H–L).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cxcr4b WT standard conditions Prim-15 olfactory pit ISH
WT + MO2-tfap2c + MO4-tfap2a standard conditions Prim-15 Not Detected olfactory pit ISH
dlx3b WT standard conditions 75%-epiboly preplacodal ectoderm ISH
WT + MO2-tfap2c + MO4-tfap2a standard conditions 75%-epiboly preplacodal ectoderm ISH
foxe3 WT standard conditions Prim-15 lens ISH
WT + MO2-tfap2c + MO4-tfap2a standard conditions Prim-15 Not Detected lens ISH
foxi1 WT standard conditions 75%-epiboly preplacodal ectoderm ISH
WT + MO2-tfap2c + MO4-tfap2a standard conditions 75%-epiboly preplacodal ectoderm ISH
gata3 WT standard conditions 75%-epiboly preplacodal ectoderm ISH
WT + MO2-tfap2c + MO4-tfap2a standard conditions 75%-epiboly preplacodal ectoderm ISH
neurod1 WT standard conditions 10-13 somites anterior lateral line system ISH
10-13 somites trigeminal placode ISH
WT + MO2-tfap2c + MO4-tfap2a standard conditions 10-13 somites Not Detected anterior lateral line system ISH
10-13 somites Not Detected trigeminal placode ISH
pax2a WT standard conditions 5-9 somites epibranchial ganglion ISH
5-9 somites otic placode ISH
WT + MO2-tfap2c + MO4-tfap2a standard conditions 5-9 somites Not Detected epibranchial ganglion ISH
5-9 somites Not Detected otic placode ISH
pax8 WT standard conditions Bud epibranchial ganglion ISH
Bud otic placode ISH
WT + MO2-tfap2c + MO4-tfap2a standard conditions Bud Not Detected epibranchial ganglion ISH
Bud Not Detected otic placode ISH
phox2a WT standard conditions Prim-15 epibranchial ganglion ISH
WT + MO2-tfap2c + MO4-tfap2a standard conditions Prim-15 Not Detected epibranchial ganglion ISH
pitx3 WT standard conditions 5-9 somites adenohypophyseal placode ISH
5-9 somites lens placode ISH
5-9 somites olfactory field ISH
WT + MO2-tfap2c + MO4-tfap2a standard conditions 5-9 somites Not Detected adenohypophyseal placode ISH
5-9 somites Not Detected lens placode ISH
5-9 somites Not Detected olfactory field ISH
tfap2a WT standard conditions 75%-epiboly preplacodal ectoderm ISH
tfap2ats213/ts213 standard conditions 75%-epiboly preplacodal ectoderm ISH
tfap2c WT standard conditions 75%-epiboly preplacodal ectoderm ISH
tfap2ats213/ts213 standard conditions 75%-epiboly preplacodal ectoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-dlx3b + MO2-foxi1 standard conditions Prim-15 otic vesicle hypoplastic, abnormal
WT + MO2-tfap2c + MO4-tfap2a standard conditions Bud ectodermal placode development decreased occurrence, abnormal
Bud epibranchial ganglion aplastic, abnormal
Bud otic placode aplastic, abnormal
Bud otic placode formation decreased occurrence, abnormal
5-9 somites adenohypophyseal placode aplastic, abnormal
5-9 somites ectodermal placode development decreased occurrence, abnormal
5-9 somites epibranchial ganglion aplastic, abnormal
5-9 somites lens placode aplastic, abnormal
5-9 somites olfactory field aplastic, abnormal
5-9 somites olfactory placode development decreased occurrence, abnormal
5-9 somites otic placode aplastic, abnormal
10-13 somites anterior lateral line aplastic, abnormal
10-13 somites ectodermal placode development decreased occurrence, abnormal
10-13 somites trigeminal placode aplastic, abnormal
Prim-15 ectodermal placode development decreased occurrence, abnormal
Prim-15 epibranchial ganglion aplastic, abnormal
Prim-15 lens aplastic, abnormal
Prim-15 olfactory pit aplastic, abnormal
Prim-15 olfactory placode development decreased occurrence, abnormal
Prim-15 otic vesicle hypoplastic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 373(1), Bhat, N., Kwon, H.J., and Riley, B.B., A gene network that coordinates preplacodal competence and neural crest specification in zebrafish, 107-117, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.