ZFIN Figure: Bhat et al., 2013, Fig. 1

ZFIN ID: ZDB-FIG-130123-65

ZFIN ID: ZDB-FIG-130123-65

Bhat et al., 2013 - A gene network that coordinates preplacodal competence and neural crest specification in zebrafish. Developmental Biology 373(1):107-117 Full text @ Dev. Biol.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	cxcr4b dlx3b foxe3 foxi1 gata3 neurod1 pax2a pax8 phox2a pitx3 tfap2a tfap2c
Fish:	WT tfap2a^ts213/ts213 WT + MO2-tfap2c + MO4-tfap2a
Knockdown Reagents:	MO2-tfap2c MO4-tfap2a
Anatomical Terms:	adenohypophyseal placode anterior lateral line system epibranchial ganglion lens lens placode olfactory field olfactory pit otic placode preplacodal ectoderm trigeminal placode
Stage Range:	75%-epiboly to Prim-15

PHENOTYPE:

Fish:	WT + MO1-dlx3b + MO2-foxi1 WT + MO2-tfap2c + MO4-tfap2a
Knockdown Reagents:	MO1-dlx3b MO2-foxi1 MO2-tfap2c MO4-tfap2a
Observed In:	adenohypophyseal placode anterior lateral line ectodermal placode development epibranchial ganglion lens lens placode olfactory field olfactory pit olfactory placode development otic placode otic placode formation otic vesicle trigeminal placode
Stage Range:	Bud to Prim-15

Fig. 1 Knockdown of tfap2a/c impairs early placode development. A–G, Expression of early placodal markers in control embryos and tfap2a/c morphants. Expression of pitx3 at 12.5 hpf marks pituitary/olfactory/lens precursors (A); cxcr4b marks the olfactory pit at 30 hpf (B); foxe3 marks the lens at 30 hpf (C); neuroD marks the trigeminal (tg) and anterior lateral line (al) at 14 hpf (D); pax8 at 10 hpf (E) and pax2a at 12 hpf (F) mark otic/epibranchial precursors; phox2a marks epibranchial ganglia (eg) at 30 hpf (G). H–J, Expression of competence factors foxi1 (H) and gata3 (I), and PPE marker dlx3b (J), at 8.5 hpf in control embryos and tfap2a/c morphants. K and L, Expression of tfap2a (K) and tfap2c (L) at 8.5 hpf in control embryos and tfap2a^-/- (lockjaw) mutants. M, otic vesicle at 30 hpf in a control embryo, tfap2a/c morphant, and embryos injected with sub-effective doses (0.5 ng) of foxi1-MO, dlx3b-MO or both foxi1-MO and dlx3b-MO. Sub-effective doses of these MO have little or no effect by themselves, but when combined have synergistic effects that phenocopy the tfap2a/c morphant. Images show dorsal views with anterior up (A, D–F), lateral views with anterior to the left (B, C, G, M) or lateral views with dorsal to the right (H–L).

Gene Expression Details

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay
cxcr4b	WT	standard conditions	Prim-15		olfactory pit	ISH
	WT + MO2-tfap2c + MO4-tfap2a	standard conditions	Prim-15	Not Detected	olfactory pit	ISH
dlx3b	WT	standard conditions	75%-epiboly		preplacodal ectoderm	ISH
	WT + MO2-tfap2c + MO4-tfap2a	standard conditions	75%-epiboly		preplacodal ectoderm	ISH
foxe3	WT	standard conditions	Prim-15		lens	ISH
	WT + MO2-tfap2c + MO4-tfap2a	standard conditions	Prim-15	Not Detected	lens	ISH
foxi1	WT	standard conditions	75%-epiboly		preplacodal ectoderm	ISH
	WT + MO2-tfap2c + MO4-tfap2a	standard conditions	75%-epiboly		preplacodal ectoderm	ISH
gata3	WT	standard conditions	75%-epiboly		preplacodal ectoderm	ISH
	WT + MO2-tfap2c + MO4-tfap2a	standard conditions	75%-epiboly		preplacodal ectoderm	ISH
neurod1	WT	standard conditions	10-13 somites		anterior lateral line system	ISH
			10-13 somites		trigeminal placode	ISH
	WT + MO2-tfap2c + MO4-tfap2a	standard conditions	10-13 somites	Not Detected	anterior lateral line system	ISH
			10-13 somites	Not Detected	trigeminal placode	ISH
pax2a	WT	standard conditions	5-9 somites		epibranchial ganglion	ISH
			5-9 somites		otic placode	ISH
	WT + MO2-tfap2c + MO4-tfap2a	standard conditions	5-9 somites	Not Detected	epibranchial ganglion	ISH
			5-9 somites	Not Detected	otic placode	ISH
pax8	WT	standard conditions	Bud		epibranchial ganglion	ISH
			Bud		otic placode	ISH
	WT + MO2-tfap2c + MO4-tfap2a	standard conditions	Bud	Not Detected	epibranchial ganglion	ISH
			Bud	Not Detected	otic placode	ISH
phox2a	WT	standard conditions	Prim-15		epibranchial ganglion	ISH
	WT + MO2-tfap2c + MO4-tfap2a	standard conditions	Prim-15	Not Detected	epibranchial ganglion	ISH
pitx3	WT	standard conditions	5-9 somites		adenohypophyseal placode	ISH
			5-9 somites		lens placode	ISH
			5-9 somites		olfactory field	ISH
	WT + MO2-tfap2c + MO4-tfap2a	standard conditions	5-9 somites	Not Detected	adenohypophyseal placode	ISH
			5-9 somites	Not Detected	lens placode	ISH
			5-9 somites	Not Detected	olfactory field	ISH
tfap2a	WT	standard conditions	75%-epiboly		preplacodal ectoderm	ISH
	tfap2a^ts213/ts213	standard conditions	75%-epiboly		preplacodal ectoderm	ISH
tfap2c	WT	standard conditions	75%-epiboly		preplacodal ectoderm	ISH
	tfap2a^ts213/ts213	standard conditions	75%-epiboly		preplacodal ectoderm	ISH

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
WT + MO1-dlx3b + MO2-foxi1	standard conditions	Prim-15	otic vesicle hypoplastic, abnormal
WT + MO2-tfap2c + MO4-tfap2a	standard conditions	Bud	ectodermal placode development decreased occurrence, abnormal
		Bud	epibranchial ganglion aplastic, abnormal
		Bud	otic placode aplastic, abnormal
		Bud	otic placode formation decreased occurrence, abnormal
		5-9 somites	adenohypophyseal placode aplastic, abnormal
		5-9 somites	ectodermal placode development decreased occurrence, abnormal
		5-9 somites	epibranchial ganglion aplastic, abnormal
		5-9 somites	lens placode aplastic, abnormal
		5-9 somites	olfactory field aplastic, abnormal
		5-9 somites	olfactory placode development decreased occurrence, abnormal
		5-9 somites	otic placode aplastic, abnormal
		10-13 somites	anterior lateral line aplastic, abnormal
		10-13 somites	ectodermal placode development decreased occurrence, abnormal
		10-13 somites	trigeminal placode aplastic, abnormal
		Prim-15	ectodermal placode development decreased occurrence, abnormal
		Prim-15	epibranchial ganglion aplastic, abnormal
		Prim-15	lens aplastic, abnormal
		Prim-15	olfactory pit aplastic, abnormal
		Prim-15	olfactory placode development decreased occurrence, abnormal
		Prim-15	otic vesicle hypoplastic, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Reprinted from Developmental Biology, 373(1), Bhat, N., Kwon, H.J., and Riley, B.B., A gene network that coordinates preplacodal competence and neural crest specification in zebrafish, 107-117, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.

Acknowledgments:

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 373(1), Bhat, N., Kwon, H.J., and Riley, B.B., A gene network that coordinates preplacodal competence and neural crest specification in zebrafish, 107-117, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.