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ZFIN ID: ZDB-FIG-130123-66
Bhat et al., 2013 - A gene network that coordinates preplacodal competence and neural crest specification in zebrafish. Developmental Biology   373(1):107-117 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: 1-4 somites to Prim-15
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: 10-13 somites to Prim-15

Fig. 2 Misexpression of tfap2a promotes overproduction of placodes. A-L, Markers of placodal derivatives, including pitx3 in the anterior pituitary at 30 hpf (A and B, arrows), cxcr4b in the olfactory pit at 24 hpf (C and D, circled), Isl1 in trigeminal placode at 14 hpf (E and F), isl1 in cranial ganglia at 30 hpf (G and H, trigeminal ganglion indicated by arrows), pax2a in the otic/epibranchial placodes at 14 hpf (I and J, brackets indicate otic domain), and the otic vesicle at 30 hpf (K and L) in control embryos and hs:tfap2a/+ transgenic embryos. All indicated placodal derivatives are enlarged following activation of hs:tfap2a at 7 hpf. M–T, expression of competence factors tfap2c (M and N), foxi1 (O and P) and gata3 (Q and R, otic domain indicated by brackets) at 14 hpf, and the general PPE marker dlx3b at 11 hpf (S and T) in control embryos and hs:tfap2a embryos. Activation of hs:tfap2a at 7 hpf results in upregulated and expanded expression of all of these genes in placodal tissues. Images show lateral views with anterior to the left (C, D, G-L), dorsal views with anterior up (E, F, M-T), or facial views of the front of the head (A and B).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cxcr4b WT standard conditions Prim-5 olfactory placode ISH
x24Tg heat shock Prim-5 olfactory placode ISH
dlx3b WT standard conditions 1-4 somites neurogenic field ISH
x24Tg heat shock 1-4 somites neurogenic field ISH
foxi1 WT standard conditions 10-13 somites neurogenic field ISH
x24Tg heat shock 10-13 somites neurogenic field ISH
gata3 WT standard conditions 10-13 somites neurogenic field ISH
10-13 somites otic placode ISH
x24Tg heat shock 10-13 somites neurogenic field ISH
10-13 somites otic placode ISH
isl1 WT standard conditions Prim-15 cranial ganglion ISH
Prim-15 trigeminal ganglion ISH
x24Tg heat shock Prim-15 cranial ganglion ISH
Prim-15 trigeminal ganglion ISH
pax2a WT standard conditions 10-13 somites epibranchial field ISH
10-13 somites otic placode ISH
x24Tg heat shock 10-13 somites epibranchial field ISH
10-13 somites otic placode ISH
pitx3 WT standard conditions Prim-15 adenohypophyseal placode ISH
x24Tg heat shock Prim-15 adenohypophyseal placode ISH
tfap2c WT standard conditions 10-13 somites neurogenic field ISH
x24Tg heat shock 10-13 somites neurogenic field ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
x24Tg heat shock 10-13 somites epibranchial placode increased size, abnormal
10-13 somites otic placode increased size, abnormal
10-13 somites trigeminal placode increased size, abnormal
Prim-5 olfactory pit increased size, abnormal
Prim-15 adenohypophyseal placode increased size, abnormal
Prim-15 otic vesicle increased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 373(1), Bhat, N., Kwon, H.J., and Riley, B.B., A gene network that coordinates preplacodal competence and neural crest specification in zebrafish, 107-117, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.