ZFIN ID: ZDB-FIG-130123-71
Bhat et al., 2013 - A gene network that coordinates preplacodal competence and neural crest specification in zebrafish. Developmental Biology   373(1):107-117 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage Range: Bud to 5-9 somites
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Bud to 5-9 somites

Fig. 7 Misexpression of tfap2a rescues PPE and NC in Bmp-blocked embryos. Expression of PPE markers six4.1 (A) and dlx3b (B) at 10 hpf, NC marker foxd3 at 12 hpf (C), neural plate marker krox20 at 12 hpf (D), competence factors foxi1 (E) and gata3 (F) at 10 hpf, epidermal marker p63 at 10 hpf (G) and Bmp-feedback inhibitor sizzled at 10 hpf (H) in control embryos, embryos treated with 200 μM DM from 4 hpf, or hs:tfap2a/+transgenic embryos treated with 200 μM DM from 4 hpf and heat shocked at 6 hpf. Images show lateral views with dorsal to the right and anterior up. Insets in (A) show ventral views of corresponding specimens. Misexpression of tfap2a in DM-treated embryos rescues PPE and NC markers while causing slight disruption in stripes of krox20, but does not restore expression of other competence factors, p63 or sizzled.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx3b WT control Bud preplacodal ectoderm ISH
WT chemical treatment: pharmaceutical Bud Not Detected preplacodal ectoderm ISH
x24Tg heat shock, chemical treatment: pharmaceutical Bud preplacodal ectoderm ISH
egr2b WT control 5-9 somites neural plate ISH
WT chemical treatment: pharmaceutical 5-9 somites neural plate ISH
x24Tg heat shock, chemical treatment: pharmaceutical 5-9 somites neural plate ISH
foxd3 WT control 5-9 somites neural crest ISH
WT chemical treatment: pharmaceutical 5-9 somites Not Detected neural crest ISH
x24Tg heat shock, chemical treatment: pharmaceutical 5-9 somites neural crest ISH
foxi1 WT control Bud neurogenic field ISH
WT chemical treatment: pharmaceutical Bud Not Detected neurogenic field ISH
x24Tg heat shock, chemical treatment: pharmaceutical Bud Not Detected neurogenic field ISH
gata3 WT control Bud neurogenic field ISH
WT chemical treatment: pharmaceutical Bud Not Detected neurogenic field ISH
x24Tg heat shock, chemical treatment: pharmaceutical Bud Not Detected neurogenic field ISH
six4b WT control Bud preplacodal ectoderm ISH
WT chemical treatment: pharmaceutical Bud Not Detected preplacodal ectoderm ISH
x24Tg heat shock, chemical treatment: pharmaceutical Bud preplacodal ectoderm ISH
tp63 WT control Bud ectoderm ISH
WT chemical treatment: pharmaceutical Bud Not Detected ectoderm ISH
x24Tg heat shock, chemical treatment: pharmaceutical Bud Not Detected ectoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: pharmaceutical Bud whole organism wholly dorsalized, abnormal
5-9 somites whole organism wholly dorsalized, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 373(1), Bhat, N., Kwon, H.J., and Riley, B.B., A gene network that coordinates preplacodal competence and neural crest specification in zebrafish, 107-117, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.