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ZFIN ID: ZDB-FIG-111017-5
Ramachandran et al., 2011 - Ascl1a/Dkk/ beta -catenin signaling pathway is necessary and glycogen synthase kinase-3 beta inhibition is sufficient for zebrafish retina regeneration. Proceedings of the National Academy of Sciences of the United States of America   108(38):15858-63 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage: Adult

Fig. 1 Ascl1a inhibits the expression of dkk genes during retina regeneration. (A and B) Injury-dependent regulation of Wnt signaling component mRNAs. (C) Double in situ hybridization shows mutually exclusive ascl1a and dkk1b gene expression. (D) ascl1a and dkk1b expression in FACS-purified MG and non-MG from injured retinas. Values are relative to uninjured retina. *P < 0.009. (E and F) Ascl1a knockdown prevents injury-dependent dkk gene suppression. (F) Quantification of E by qPCR. Values are relative to uninjured retina. *P < 0.0001. (G) In situ hybridization showing Ascl1a knockdown relieves injury-dependent dkk suppression. Boxed region in low-magnification image is shown in higher magnification in the row below. Arrows point to ascl1a+/dkk1b+ cells. White dots identify autofluorescence in ONL. (H and I) Injection of zebrafish embryos with dkk1b:gfp-luciferase reporter and increasing amounts of ascl1a mRNA (H) or ascl1a-targeting MO (I). *P < 0.005. (J) Lin-28 knockdown differentially affects injury-dependent dkk gene suppression. (K and L) Dkk1b overexpression inhibits cell proliferation at 4 dpi. *P < 0.003. (Scale bars, 10 μm.) ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ascl1a WT standard conditions Adult Not Detected retinal inner nuclear layer ISH
Adult Not Detected retinal outer nuclear layer ISH
WT physical alteration: anatomical structure Adult retinal inner nuclear layer ISH
Adult retinal outer nuclear layer ISH
mi4Tg physical alteration: anatomical structure Adult Muller cell RTPCR
Adult retina RTPCR
nt11Tg standard conditions Adult Not Detected Muller cell RTPCR
Adult Not Detected retina RTPCR
dkk1a WT standard conditions Adult retinal inner nuclear layer ISH
Adult retinal outer nuclear layer ISH
WT physical alteration: anatomical structure Adult Not Detected retinal inner nuclear layer ISH
Adult Not Detected retinal outer nuclear layer ISH
mi4Tg physical alteration: anatomical structure Adult Muller cell RTPCR
Adult retina RTPCR
nt11Tg standard conditions Adult Not Detected Muller cell RTPCR
Adult retina RTPCR
Adult Not Detected retina RTPCR
dkk1b mi4Tg physical alteration: anatomical structure Adult Not Detected Muller cell RTPCR
Adult retina RTPCR
mi4Tg + MO1-ascl1a physical alteration: anatomical structure Adult Muller cell RTPCR
mi4Tg + MO1-lin28a physical alteration: anatomical structure Adult Muller cell RTPCR
mi4Tg + MO2-ascl1a physical alteration: anatomical structure Adult Muller cell RTPCR
mi4Tg + MO2-lin28a physical alteration: anatomical structure Adult Muller cell RTPCR
nt11Tg standard conditions Adult Muller cell RTPCR
Adult retina RTPCR
dkk2 mi4Tg physical alteration: anatomical structure Adult Not Detected Muller cell RTPCR
Adult retina RTPCR
mi4Tg + MO1-ascl1a physical alteration: anatomical structure Adult Muller cell RTPCR
mi4Tg + MO1-lin28a physical alteration: anatomical structure Adult Muller cell RTPCR
mi4Tg + MO2-ascl1a physical alteration: anatomical structure Adult Muller cell RTPCR
mi4Tg + MO2-lin28a physical alteration: anatomical structure Adult Muller cell RTPCR
nt11Tg standard conditions Adult retina RTPCR
dkk3a mi4Tg physical alteration: anatomical structure Adult Not Detected Muller cell RTPCR
Adult retina RTPCR
mi4Tg + MO1-ascl1a physical alteration: anatomical structure Adult Muller cell RTPCR
mi4Tg + MO1-lin28a physical alteration: anatomical structure Adult Not Detected Muller cell RTPCR
mi4Tg + MO2-ascl1a physical alteration: anatomical structure Adult Muller cell RTPCR
mi4Tg + MO2-lin28a physical alteration: anatomical structure Adult Not Detected Muller cell RTPCR
nt11Tg standard conditions Adult Not Detected Muller cell RTPCR
Adult retina RTPCR
dkk3b mi4Tg physical alteration: anatomical structure Adult Not Detected Muller cell RTPCR
mi4Tg + MO1-ascl1a physical alteration: anatomical structure Adult Muller cell RTPCR
mi4Tg + MO1-lin28a physical alteration: anatomical structure Adult Muller cell RTPCR
mi4Tg + MO2-ascl1a physical alteration: anatomical structure Adult Muller cell RTPCR
mi4Tg + MO2-lin28a physical alteration: anatomical structure Adult Muller cell RTPCR
nt11Tg standard conditions Adult Muller cell RTPCR
fzd2 mi4Tg physical alteration: anatomical structure Adult Muller cell RTPCR
Adult retina RTPCR
nt11Tg standard conditions Adult Not Detected Muller cell RTPCR
Adult Not Detected retina RTPCR
fzd3a mi4Tg physical alteration: anatomical structure Adult Muller cell RTPCR
Adult retina RTPCR
nt11Tg standard conditions Adult Not Detected Muller cell RTPCR
Adult retina RTPCR
fzd8a mi4Tg physical alteration: anatomical structure Adult Muller cell RTPCR
Adult retina RTPCR
nt11Tg standard conditions Adult Not Detected Muller cell RTPCR
Adult retina RTPCR
fzd8b mi4Tg physical alteration: anatomical structure Adult Muller cell RTPCR
Adult retina RTPCR
nt11Tg standard conditions Adult Not Detected Muller cell RTPCR
Adult retina RTPCR
fzd9a mi4Tg physical alteration: anatomical structure Adult Muller cell RTPCR
Adult retina RTPCR
nt11Tg standard conditions Adult Not Detected Muller cell RTPCR
Adult retina RTPCR
gapdh mi4Tg physical alteration: anatomical structure Adult Muller cell RTPCR
Adult retina RTPCR
nt11Tg standard conditions Adult Muller cell RTPCR
Adult retina RTPCR
wnt2 mi4Tg physical alteration: anatomical structure Adult Not Detected Muller cell RTPCR
Adult Not Detected retina RTPCR
nt11Tg standard conditions Adult Muller cell RTPCR
Adult retina RTPCR
wnt2ba mi4Tg physical alteration: anatomical structure Adult Muller cell RTPCR
Adult retina RTPCR
nt11Tg standard conditions Adult Not Detected Muller cell RTPCR
Adult Not Detected retina RTPCR
wnt3 mi4Tg physical alteration: anatomical structure Adult Not Detected Muller cell RTPCR
Adult retina RTPCR
nt11Tg standard conditions Adult Muller cell RTPCR
Adult retina RTPCR
wnt4 mi4Tg physical alteration: anatomical structure Adult Muller cell RTPCR
Adult retina RTPCR
nt11Tg standard conditions Adult Not Detected Muller cell RTPCR
Adult Not Detected retina RTPCR
wnt8b mi4Tg physical alteration: anatomical structure Adult Muller cell RTPCR
Adult retina RTPCR
nt11Tg standard conditions Adult Not Detected Muller cell RTPCR
Adult Not Detected retina RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA