Ascl1a/Dkk/ beta -catenin signaling pathway is necessary and glycogen synthase kinase-3 beta inhibition is sufficient for zebrafish retina regeneration

Ramachandran, R., Zhao, X.F., and Goldman, D.
Proceedings of the National Academy of Sciences of the United States of America   108(38): 15858-63 (Journal)
Registered Authors
Goldman, Dan
pyrvinium, XAV939, transgenic zebrafish, heat shock, frizzled
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Basic Helix-Loop-Helix Transcription Factors/genetics
  • Basic Helix-Loop-Helix Transcription Factors/metabolism
  • Cell Dedifferentiation/drug effects
  • Cell Dedifferentiation/genetics
  • Cell Proliferation/drug effects
  • Enzyme Inhibitors/pharmacology
  • Gene Expression Profiling
  • Gene Knockdown Techniques
  • Glycogen Synthase Kinase 3/antagonists & inhibitors*
  • Glycogen Synthase Kinase 3/genetics
  • Glycogen Synthase Kinase 3/metabolism
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • In Situ Hybridization
  • Intercellular Signaling Peptides and Proteins/genetics
  • Intercellular Signaling Peptides and Proteins/metabolism
  • Microscopy, Fluorescence
  • Neuroglia/cytology
  • Neuroglia/drug effects
  • Neuroglia/metabolism
  • Regeneration*
  • Retina/metabolism
  • Retina/physiology*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction*
  • Stem Cells/drug effects
  • Stem Cells/metabolism
  • Wnt4 Protein/genetics
  • Wnt4 Protein/metabolism
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zebrafish/physiology
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • beta Catenin/genetics
  • beta Catenin/metabolism
21911394 Full text @ Proc. Natl. Acad. Sci. USA
Key to successful retina regeneration in zebrafish are Müller glia (MG) that respond to retinal injury by dedifferentiating into a cycling population of retinal progenitors. Although recent studies have identified several genes involved in retina regeneration, the signaling mechanisms underlying injury-dependent MG proliferation have remained elusive. Here we report that canonical Wnt signaling controls the proliferation of MG-derived retinal progenitors. We found that injury-dependent induction of Ascl1a suppressed expression of the Wnt signaling inhibitor, Dkk, and induced expression of the Wnt ligand, Wnt4a. Genetic and pharmacological inhibition of Wnt signaling suppressed injury-dependent proliferation of MG-derived progenitors. Remarkably, in the uninjured retina, glycogen synthase kinase-3β (GSK-3β) inhibition was sufficient to stimulate MG dedifferentiation and the formation of multipotent retinal progenitors that were capable of differentiating into all major retinal cell types. Importantly, Ascl1a expression was found to contribute to the multipotential character of these progenitors. Our data suggest that Wnt signaling and GSK-3β inhibition, in particular, are crucial for successful retina regeneration.
Genes / Markers
Show all Figures
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes