ZFIN Gene: ascl1a

ZFIN ID: ZDB-GENE-980526-90

ZFIN ID: ZDB-GENE-980526-90

Gene Name:

achaete-scute family bHLH transcription factor 1a

Gene Symbol:

ascl1a Nomenclature History

Previous Names:

asha, Zash-1a (1), zash1a, id:ibd5085 (1), pia, pituitary absent, zasha

Gene Type:

protein_coding_gene

Location:

Chr: 4 Mapping Details/Browsers

Description:

Exhibits sequence-specific DNA binding activity. Predicted to contribute to DNA-binding transcription factor activity. Involved in several processes, including animal organ development; epithelial cell differentiation; and regulation of gene expression. Predicted to localize to RNA polymerase II transcription regulator complex. Is expressed in several structures, including digestive system; nervous system; neural tube; neuronal stem cell; and optic cup. Human ortholog(s) of this gene implicated in congenital central hypoventilation syndrome. Orthologous to human ASCL1 (achaete-scute family bHLH transcription factor 1).

Genome Resources:

Alliance (1), Gene:30466 (1), Ensembl(GRCz11):ENSDARG00000038386 (2)

GENE EXPRESSION
All Expression Data:	134 figures from 81 publications
Directly Submitted Expression Data:	3 figures (4 images) from Kudoh et al., 2001 [ibd5085]
	6 figures (16 images) from Thisse et al., 2005 [eu887]
Wild-type Stages, Structures:	Blastula:Sphere (4.0h-4.33h) to Adult (90d-730d, breeding adult)
	adenohypophyseal placode adenohypophysis anterior neural rod ventral region basal plate midbrain region brain caudal tuberal nucleus central nervous system cerebellum ciliary marginal zone diencephalon GABAergic neuron diencephalon dorso-rostral cluster enteric neuron epiphysis eye forebrain dorsal region forebrain ventral region forebrain gut head hindbrain dorsal region hindbrain ventral region hindbrain hindbrain neural keel dorsal region hypophysis hypothalamus intestinal epithelium intestine lip epithelium taste bud midbrain posterior region midbrain ventral region midbrain midbrain hindbrain boundary midbrain hindbrain boundary neural keel dorsal region midbrain hindbrain boundary neural keel midbrain hindbrain boundary neural rod neural plate neural tube neuroblast neurohypophysis neuronal stem cell olfactory epithelium optic cup optic tectum ventricular zone optic tectum optic vesicle oral epithelium pharyngeal arch taste bud pharynx epithelium presumptive diencephalon presumptive neural retina pretectum retina central region retina peripheral region retina retinal inner nuclear layer retinal neural layer retinal outer nuclear layer rhombomere rhombomere 2 rhombomere 3 spinal cord spinal cord neural tube superior cervical ganglion tegmentum telencephalon neuroblast (sensu Vertebrata) telencephalon ventricular zone telencephalon thalamus anterior region third ventricle medial region unspecified ventral hypothalamic zone ventral telencephalon GABAergic neuron ventral telencephalon ventral thalamus ventricular zone whole organism
High Throughput Expression:	GEO (1) , Expression Atlas (1)

MUTATIONS AND SEQUENCE TARGETING REAGENTS

Allele	Type	Localization	Consequence	Mutagen	Suppliers
ascl1a_unspecified	Unspecified	Unknown	Unknown
t25215	Point Mutation	Unknown	Premature Stop	ENU

Sequence Targeting Reagents

Targeting Reagent	Created Alleles	Publications
CRISPR1-ascl1a		1
CRISPR2-ascl1a		1
CRISPR3-ascl1a		1
CRISPR4-ascl1a		1
CRISPR5-ascl1a		1
CRISPR6-ascl1a		1
MO1-ascl1a	N/A	15
MO2-ascl1a	N/A	13
MO3-ascl1a	N/A	6
MO4-ascl1a	N/A	1

PHENOTYPE

Data:

14 figures from 8 publications

Observed in:

DISEASE ASSOCIATED WITH ascl1a HUMAN ORTHOLOG

Disease Ontology Term	Multi-Species Data	OMIM Term	OMIM Phenotype ID
congenital central hypoventilation syndrome	Alliance	Central hypoventilation syndrome, congenital	209880
		Haddad syndrome	209880

DISEASE ASSOCIATED WITH ascl1a VIA EXPERIMENTAL MODELS No data available

GENE ONTOLOGY

Ontology	GO Term
Biological Process	positive regulation of transcription, DNA-templated (more)
Cellular Component	nucleus (more)
Molecular Function	sequence-specific DNA binding (more)

GO Terms (all 30)

PROTEIN FAMILIES, DOMAINS AND SITES No links to external sites available

TRANSCRIPTS
Type	Name	Length (nt)	Analysis
mRNA	ascl1a-201 (1)	1977	Select Tool ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT

Browsers: UCSC, NCBI, Ensembl, ZFIN

INTERACTIONS AND PATHWAYS

ANTIBODIES

Name	Type	Isotype	Host Organism	Assay	Source	Publications
Ab1-ascl1	polyclonal		Rabbit	IHC	Sigma-Aldrich	3

PLASMIDS No data available

CONSTRUCTS WITH SEQUENCES FROM ascl1a

Construct	Regulatory Regions	Coding Sequences	Species	Tg Lines	Publications
Tg(-4.5ascl1a:EGFP)	ascl1a	EGFP	Danio rerio	1	2
Tg(-6ascl1a:EGFP)	ascl1a	EGFP	Danio rerio	1	2
Tg(ascl1a:EGFP)	ascl1a	EGFP	Danio rerio	1	5
Tg(hsp70l:FLAG-MYC-ascl1a,-1.0Cau.Tuba1:GFP)	hsp70l	ascl1a, FLAG, GFP, MYC	Carassius auratus, Danio rerio	1	1
Tg2(-6ascl1a:EGFP)	ascl1a	EGFP	Danio rerio	1	2
Tg3(-6ascl1a:EGFP)	ascl1a	EGFP	Danio rerio	1	2

MARKER RELATIONSHIPS

ascl1a Contained in:	[BAC] CH211-260P3 (1), DKEY-264K15 (1) (order this)
ascl1a Encodes:	[EST] eu887 (1), ibd5085 (1) [cDNA] MGC:109810 (1)

SEQUENCE INFORMATION

Type	Accession #	Length (nt/aa)	Analysis
RNA	RefSeq:NM_131219 (1)	1561 nt	Select Tool ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Genomic	GenBank:BX511171	166242 nt	Select Tool MegaBLAST Ensembl
Polypeptide	UniProtKB:Q90259 (1)	196 aa	Select Tool ZFIN BLAST SIB BLAST Ensembl Protein Blast

Sequence Information (all 16)

ORTHOLOGY for ascl1a ( Chr: 4 )

CITATIONS (179)

{{control.fieldName}} Edit

Attributions

Add new Alias

Attributions for Alias: {{control.newAlias}}

Delete Alias: