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ZFIN ID: ZDB-FIG-150318-13
Gerlach et al., 2014 - Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta. Developmental Biology   396(2):183-200 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 8 Ectopic expression of podocyte genes and early developmental transcription factors in the pronephros is not a general consequence of embryonic defects in fluid flow. WISH was used to assess gene expression using antisense riboprobes to detect wt1a, pax2a, or podxl (purple) in (A) wild-types, (B) prkCI morphants, (C) ift88 morphants, (D) tnnt2a morphants, (E) double ift88/tnnt2a morphants, and (F) mpp5a morphants. (A–E) Knockdown of prkCI was the only circumstance that led to high levels of ectopic expression of podocyte genes in the pronephros tubule. (F) (Top row) Most mpp5a morphants displayed no ectopic pronephros expression, but (bottom row) a subset (<10%) displayed low levels of ectopic wt1a, pax2a, or podxl in the tubule and also diffuse ectopic expression.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
pax2a TU control Protruding-mouth pronephric tubule ISH
TU + MO1-mpp5a standard conditions Protruding-mouth pronephric tubule ISH
TU + MO1-prkci + MO1-prkcz standard conditions Protruding-mouth pronephric proximal convoluted tubule ISH
Protruding-mouth pronephric proximal straight tubule ISH
Protruding-mouth pronephric tubule ISH
TU + MO1-tnnt2a standard conditions Protruding-mouth pronephric tubule ISH
TU + MO1-tnnt2a + MO2-ift88 standard conditions Protruding-mouth pronephric tubule ISH
TU + MO2-ift88 standard conditions Protruding-mouth pronephric tubule ISH
podxl TU control Protruding-mouth pronephric podocyte ISH
TU + MO1-mpp5a standard conditions Protruding-mouth pronephric podocyte ISH
TU + MO1-prkci + MO1-prkcz standard conditions Protruding-mouth pronephric podocyte ISH
Protruding-mouth pronephric proximal convoluted tubule ISH
Protruding-mouth pronephric proximal straight tubule ISH
TU + MO1-tnnt2a standard conditions Protruding-mouth pronephric podocyte ISH
TU + MO1-tnnt2a + MO2-ift88 standard conditions Protruding-mouth pronephric podocyte ISH
TU + MO2-ift88 standard conditions Protruding-mouth pronephric podocyte ISH
wt1a TU control Protruding-mouth pronephric podocyte ISH
TU + MO1-mpp5a standard conditions Protruding-mouth pronephric podocyte ISH
TU + MO1-prkci + MO1-prkcz standard conditions Protruding-mouth pronephric podocyte ISH
Protruding-mouth pronephric proximal convoluted tubule ISH
Protruding-mouth pronephric proximal straight tubule ISH
TU + MO1-tnnt2a standard conditions Protruding-mouth pronephric podocyte ISH
TU + MO1-tnnt2a + MO2-ift88 standard conditions Protruding-mouth pronephric podocyte ISH
TU + MO2-ift88 standard conditions Protruding-mouth pronephric podocyte ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO1-mpp5a standard conditions Protruding-mouth establishment or maintenance of epithelial cell apical/basal polarity process quality, abnormal
TU + MO1-prkci + MO1-prkcz standard conditions Protruding-mouth pronephric proximal convoluted tubule cell differentiation involved in pronephros development process quality, abnormal
Protruding-mouth pronephric proximal straight tubule cell differentiation involved in pronephros development process quality, abnormal
TU + MO1-tnnt2a standard conditions Protruding-mouth heart contraction arrested, abnormal
Protruding-mouth pronephric proximal convoluted tubule cell differentiation involved in pronephros development process quality, normal
Protruding-mouth pronephric proximal straight tubule cell differentiation involved in pronephros development process quality, normal
TU + MO1-tnnt2a + MO2-ift88 standard conditions Protruding-mouth heart contraction arrested, abnormal
Protruding-mouth pronephric proximal convoluted tubule cell differentiation involved in pronephros development process quality, normal
Protruding-mouth pronephric proximal straight tubule cell differentiation involved in pronephros development process quality, normal
Protruding-mouth pronephros epithelial cilium movement involved in extracellular fluid movement arrested, abnormal
TU + MO2-ift88 standard conditions Protruding-mouth pronephric proximal convoluted tubule cell differentiation involved in pronephros development process quality, normal
Protruding-mouth pronephric proximal straight tubule cell differentiation involved in pronephros development process quality, normal
Protruding-mouth pronephros epithelial cilium movement involved in extracellular fluid movement arrested, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 396(2), Gerlach, G.F., Wingert, R.A., Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta, 183-200, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.