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ZFIN ID: ZDB-FIG-150318-11
Gerlach et al., 2014 - Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta. Developmental Biology   396(2):183-200 Full text @ Dev. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 6 Renal clearance and PCT endocytosis are abrogated in prkCI deficient embryos. Embryos were injected with 40-kDa dextran–FITC at 48 hpf, and imaged at 72, 84, and 96 hpf. Control wild-type embryos displayed renal clearance by diminution of the net fluorescent signal intensity over time, as well as dextran–FITC internalization throughout the PCT (white arrows) during its progressive morphogenesis. DoubleprkCI morphants displayed severe fluid accumulation, notably pericardial edema that remained strongly positive for the dextran–FITC conjugate label, and the PCT was not labeled by dextran endocytosis (left panels, lateral views; right panels, dorsal views, with exception of bottom right which shows a lateral/dorsal angled view).

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO1-prkci + MO1-prkcz standard conditions Protruding-mouth heart edematous, abnormal
Protruding-mouth pronephric proximal convoluted tubule endocytosis decreased occurrence, abnormal
Protruding-mouth renal filtration decreased occurrence, abnormal
Day 4 heart edematous, abnormal
Day 4 pronephric proximal convoluted tubule endocytosis decreased occurrence, abnormal
Day 4 renal filtration decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 396(2), Gerlach, G.F., Wingert, R.A., Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta, 183-200, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.