FIGURE

Fig. 6

ID

ZDB-FIG-150318-11

Publication

Gerlach et al., 2014 - Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta

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Fig. 6

Renal clearance and PCT endocytosis are abrogated in prk_CI/ζ deficient embryos. Embryos were injected with 40-kDa dextran–FITC at 48 hpf, and imaged at 72, 84, and 96 hpf. Control wild-type embryos displayed renal clearance by diminution of the net fluorescent signal intensity over time, as well as dextran–FITC internalization throughout the PCT (white arrows) during its progressive morphogenesis. Doubleprk_CI/ζ morphants displayed severe fluid accumulation, notably pericardial edema that remained strongly positive for the dextran–FITC conjugate label, and the PCT was not labeled by dextran endocytosis (left panels, lateral views; right panels, dorsal views, with exception of bottom right which shows a lateral/dorsal angled view).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU + MO1-prkci + MO1-prkcz
Knockdown Reagents:	MO1-prkci MO1-prkcz
Observed In:	heart pronephric proximal convoluted tubule endocytosis renal filtration
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 396(2), Gerlach, G.F., Wingert, R.A., Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta, 183-200, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.