ZFIN Image: Gerlach et al., 2014, Fig. 8

Image

Figure Caption

Fig. 8 Ectopic expression of podocyte genes and early developmental transcription factors in the pronephros is not a general consequence of embryonic defects in fluid flow. WISH was used to assess gene expression using antisense riboprobes to detect wt1a, pax2a, or podxl (purple) in (A) wild-types, (B) prk_CI/ζ morphants, (C) ift88 morphants, (D) tnnt2a morphants, (E) double ift88/tnnt2a morphants, and (F) mpp5a morphants. (A–E) Knockdown of prk_CI/ζ was the only circumstance that led to high levels of ectopic expression of podocyte genes in the pronephros tubule. (F) (Top row) Most mpp5a morphants displayed no ectopic pronephros expression, but (bottom row) a subset (<10%) displayed low levels of ectopic wt1a, pax2a, or podxl in the tubule and also diffuse ectopic expression.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 396(2), Gerlach, G.F., Wingert, R.A., Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta, 183-200, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.