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ZFIN ID: ZDB-FIG-150318-7
Gerlach et al., 2014 - Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta. Developmental Biology   396(2):183-200 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Adult

Fig. 2 Changes in protein localization precede lumen formation in the pronephros, and similar polarity features characterize embryonic and adult zebrafish nephron tubules. Localization of proteins during nephrogenesis in wild-type transgenic Tg(cdh17:eGFP) zebrafish embryos and in the uninjured adult kidney. Tubules not labeled with GFP (due to incompatibility of particular antibody-fixative combinations) are outlined with white dots. (A) At the 18 ss: PrkCI/ζ and actin localized to the apical membrane domain prior to the appearance of a lumen, while Na+/K+ ATPase surrounded the cell membrane and was not excluded from the apical surface. (B) When a lumen formed at the 20–22 ss: PrkCI/ζ encompassed the apical membrane domain abutting the lumen, actin was found in a punctate pattern at the apical surface presumably localizing to junctional complexes, and Na+/K+ ATPase still surrounded a majority of the cell membrane but was not present in the region where PrkCI/ζ was localized. (C) At the 28–30 ss: PrkCI/ζ demarcated the apical membrane, actin was more band-like at the apical surface yet still exhibited punctate expression at cell junctions, and Na+/K+ ATPase showed a distinct basolateral membrane localization. (D) In the adult kidney, or mesonephros, PrkCI/ζ was located at the apical surface of tubule cells, and the tight junction marker ZO-1 showed strong labeling at junctional complexes. (A–C) Scale bars, 5 µm. (D) Scale bar, 20 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nz1Tg standard conditions 14-19 somites pronephros cytoplasm IHC
20-25 somites pronephros cytoplasm IHC
26+ somites pronephros cytoplasm IHC
Adult renal tubule cytoplasm IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-atp1a1 IHC nz1Tg standard conditions 14-19 somites pronephros apical plasma membrane
IHC 14-19 somites pronephros basolateral plasma membrane
IHC 20-25 somites Not Detected pronephros apical plasma membrane
IHC 20-25 somites pronephros basolateral plasma membrane
IHC 26+ somites Not Detected pronephros apical plasma membrane
IHC 26+ somites pronephros basolateral plasma membrane
Ab1-prkcz IHC nz1Tg standard conditions 14-19 somites pronephros apical plasma membrane
IHC 14-19 somites Not Detected pronephros basolateral plasma membrane
IHC 20-25 somites pronephros apical plasma membrane
IHC 20-25 somites Not Detected pronephros basolateral plasma membrane
IHC 26+ somites pronephros apical plasma membrane
IHC 26+ somites Not Detected pronephros basolateral plasma membrane
IHC Adult renal tubule apical plasma membrane
Ab1-tjp1 IHC nz1Tg standard conditions Adult renal tubule bicellular tight junction
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 396(2), Gerlach, G.F., Wingert, R.A., Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta, 183-200, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.