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ZFIN ID: ZDB-FIG-150318-10
Gerlach et al., 2014 - Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta. Developmental Biology   396(2):183-200 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 5 prkCI/ζ knockdown does not disrupt proximodistal segmentation, but does lead to defects in PCT morphogenesis. WISH was used to assess epithelial specification and segment pattern using antisense riboprobes (purple) in wild-types (left column) and prkCI/ζ morphants (right column). (A,B) In control and prkCI/ζ morphants the proximo-distal specification of nephron segments was normal. (Lateral views, anterior to the left) (A) Podocytes were labeled with nephrin, the proximal straight tubule (PST) with trpm7, and distal late tubule (DL) with slc12a3. (B) Podocytes were labeled with wt1b, the proximal convoluted tubule (PCT) with slc20a1a, and the distal early tubule (DE) with slc12a1. (C) In prkCI/ζ morphants the PCT failed to undergo proper morphogenesis and instead remained linear, while the PCT segment in control wild-type embryos at 48 hpf and 72 hpf underwent progressive coiling morphogenesis. (Dorsal views) (D) Acridine orange labeling indicates cell death in the PCT segment of the pronephros (white arrows) in prkCI/ζ morphants, but not wild-types. (Lateral views, anterior to the left.)

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
nphs1 TU control Prim-5 pronephric podocyte ISH
TU + MO1-prkci + MO1-prkcz standard conditions Prim-5 pronephric podocyte ISH
slc12a1 TU control Prim-5 pronephric distal early tubule ISH
TU + MO1-prkci + MO1-prkcz standard conditions Prim-5 pronephric distal early tubule ISH
slc12a3 TU control Prim-5 pronephric distal late tubule ISH
TU + MO1-prkci + MO1-prkcz standard conditions Prim-5 pronephric distal late tubule ISH
slc20a1a TU control Prim-5 pronephric proximal convoluted tubule ISH
Long-pec pronephric proximal convoluted tubule ISH
Protruding-mouth pronephric proximal convoluted tubule ISH
TU + MO1-prkci + MO1-prkcz standard conditions Prim-5 pronephric proximal convoluted tubule ISH
Long-pec pronephric proximal convoluted tubule ISH
Protruding-mouth pronephric proximal convoluted tubule ISH
trpm7 TU control Prim-5 pronephric proximal straight tubule ISH
TU + MO1-prkci + MO1-prkcz standard conditions Prim-5 pronephric proximal straight tubule ISH
wt1b TU control Prim-5 pronephric podocyte ISH
TU + MO1-prkci + MO1-prkcz standard conditions Prim-5 pronephric podocyte ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO1-prkci + MO1-prkcz standard conditions Long-pec pronephric proximal convoluted tubule apoptotic, abnormal
Long-pec pronephric proximal convoluted tubule malformed, abnormal
Long-pec pronephric proximal convoluted tubule apoptotic process increased occurrence, abnormal
Long-pec pronephric proximal tubule morphogenesis decreased process quality, abnormal
Protruding-mouth pronephric proximal convoluted tubule malformed, abnormal
Protruding-mouth pronephric proximal tubule morphogenesis decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 396(2), Gerlach, G.F., Wingert, R.A., Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta, 183-200, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.