FIGURE

Fig. S4

ID

ZDB-FIG-150318-18

Publication

Gerlach et al., 2014 - Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta

Other Figures

All Figure Page

Back to All Figure Page

Fig. S4

Expression of odf3b and acetylated α-tubulin in wild-type control and prk_CI/ζ deficient embryos. WISH was used to evaluate the gene expression of odf3b (purple), a ciliogenesis marker, in wild-types (left top, middle panels) and double prk_CI/ζ morphants (right top, middle panels) at the 36 hpf stage. Wild-type embryos and double prk_CI/ζ knockdowns formed similar numbers and arrangements of odf3b-expressing cells, though occasional ectopic odf3b+ cells were observed in the proximal region of the pronephros in prk_CI/ζ morphants (boxed area, top right panel, which is enlarged in the middle right panel). Asterisk indicates region where ectopic odf3b+ cells were located. (Bottom left, right panels) IF performed on cross sections of wild-types and double prk_CI/ζ morphants at the 22–24 ss revealed that acetylated α-tubulin (light blue) was similarly present at the apical membrane of pronephros cells (labeled by GFP (green), nuclei marked with DAPI (dark blue), although double prk_CI/ζ morphants lacked Prkc (red).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 396(2), Gerlach, G.F., Wingert, R.A., Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta, 183-200, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.