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ZFIN ID: ZDB-FIG-150318-14
Gerlach et al., 2014 - Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta. Developmental Biology   396(2):183-200 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 9 Ectopic expression of early developmental transcription factors in prkCI morphants is abrogated by concomitant knockdown of pax2a. WISH was used to assess gene expression using antisense riboprobes to detect wt1a, wt1b, podxl, pax2a or cdh17 (purple), in (A) wild-types, (B) prkCI morphants, or (C) prkCI morphants with combined pax2a knockdown. Wild-types showed restricted podocyte expression of wt1a, wt1b, and podxl, while pax2a was localized to the neck and intermittent pronephros tubule cells. prkCI morphants had high ectopic expression of each gene in the pronephros tubule. Knockdown of pax2a in prkCI morphants was associated with a short proximal pronephros domain of ectopic wt1a, wt1b, and podxl expression and low cdh17 transcript levels, while the tubule segment past this short ectopic segment domain was present and marked by a robust level of cdh17 transcripts. (D) Summary of ectopic misexpression during prkCI loss of function: wild-type embryos have mutually exclusive domains of podocyte versus tubule gene expression, prkCI morphants have ectopic misexpression of transcription factors throughout the pronephros, and pax2a knockdown represses this ectopic tubule misexpression in prkCI morphants, except for the neck region of the pronephros.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cdh17 TU standard conditions Protruding-mouth pronephros ISH
TU + MO1-prkci + MO1-prkcz + MO5-pax2a standard conditions Protruding-mouth pronephros ISH
pax2a TU control Protruding-mouth pronephric tubule ISH
TU + MO1-prkci + MO1-prkcz standard conditions Protruding-mouth pronephric proximal convoluted tubule ISH
Protruding-mouth pronephric proximal straight tubule ISH
Protruding-mouth pronephric tubule ISH
podxl TU control Protruding-mouth pronephric podocyte ISH
TU + MO1-prkci + MO1-prkcz standard conditions Protruding-mouth pronephric podocyte ISH
Protruding-mouth pronephric proximal convoluted tubule ISH
Protruding-mouth pronephric tubule ISH
TU + MO1-prkci + MO1-prkcz + MO5-pax2a standard conditions Protruding-mouth pronephric podocyte ISH
Protruding-mouth Not Detected pronephric proximal convoluted tubule ISH
Protruding-mouth pronephric tubule ISH
wt1a TU control Protruding-mouth pronephric podocyte ISH
TU + MO1-prkci + MO1-prkcz standard conditions Protruding-mouth pronephric podocyte ISH
Protruding-mouth pronephric proximal convoluted tubule ISH
Protruding-mouth pronephric tubule ISH
TU + MO1-prkci + MO1-prkcz + MO5-pax2a standard conditions Protruding-mouth pronephric podocyte ISH
Protruding-mouth Not Detected pronephric proximal convoluted tubule ISH
Protruding-mouth pronephric tubule ISH
wt1b TU control Protruding-mouth pronephric podocyte ISH
TU + MO1-prkci + MO1-prkcz standard conditions Protruding-mouth pronephric podocyte ISH
Protruding-mouth pronephric proximal convoluted tubule ISH
Protruding-mouth pronephric tubule ISH
TU + MO1-prkci + MO1-prkcz + MO5-pax2a standard conditions Protruding-mouth pronephric podocyte ISH
Protruding-mouth Not Detected pronephric proximal convoluted tubule ISH
Protruding-mouth pronephric tubule ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO1-prkci + MO1-prkcz standard conditions Protruding-mouth pronephric proximal convoluted tubule cell differentiation involved in pronephros development process quality, abnormal
TU + MO1-prkci + MO1-prkcz + MO5-pax2a standard conditions Protruding-mouth pronephric proximal convoluted tubule cell differentiation involved in pronephros development process quality, normal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 396(2), Gerlach, G.F., Wingert, R.A., Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta, 183-200, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.