FIGURE

Fig. S7

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ZDB-FIG-150318-20

Publication

Gerlach et al., 2014 - Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta

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Fig. S7

prk_CI/ζ deficient embryos display reduced protein expression of the Na⁺/K⁺ ATPase in the neck region of the pronephros. Based on whole mount IF, wild-type embryos at 36 hpf exhibited Na⁺/K⁺ ATPase protein expression along the entire length of the pronephric tubule, including the proximal-most region (white bar). In contrast, double prk_CI/ζ morphants at the 36 hpf stage fail to show Na⁺/K⁺ ATPase in the proximal most region of the pronephros (line of white dots). In wild-type embryos at 72 hpf, Na⁺/K⁺ ATPase continues to demarcate the entire tubule and shows the convoluted nature of the PCT (white bar), while prk_CI/ζ deficient embryos have linear tubules that have failed to undergo normal morphogenesis. (Inset) Regions of the PCT in prk_CI/ζ morphants exhibit folds and bulges (white arrowheads).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 396(2), Gerlach, G.F., Wingert, R.A., Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta, 183-200, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.