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ZFIN ID: ZDB-FIG-150318-12
Gerlach et al., 2014 - Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta. Developmental Biology   396(2):183-200 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-25 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-25 to Protruding-mouth

Fig. 7 prkCI knockdown disrupts glomerular development and causes ectopic renal progenitor transcription factor expression. WISH was used to detect gene expression (purple) in wild-types (left column) and prkCI morphants (right column). (A,B) Left panels, lateral views; right panels, dorsal views; all embryos are shown with anterior to the left. (A) pax2a was restricted to the neck segment in wild-types, but showed ectopic podocyte and tubular expression (black arrowheads) in prkCI morphants at 36 and 72 hpf. (B) wt1a transcripts marked wild-type podocyte cells, but in prkCI morphants wt1a transcripts were also found in tubular cells (black arrowheads) and surrounding mesoderm cells. (C) wt1b and podxl were ectopically expressed in in prkCI morphants, though podxl showed ectopic expression beginning at 48 hpf and wt1b showed ectopic transcripts beginning at 72 hpf. Diffuse ectopic transcript expression (arrows) and ectopic tubule expression (black arrowheads) (dorsal views, anterior to the left).Renal clearance and PCT endocytosis are abrogated in prkCI deficient embryos. Embryos were injected with 40-kDa dextran–FITC at 48 hpf, and imaged at 72, 84, and 96 hpf. Control wild-type embryos displayed renal clearance by diminution of the net fluorescent signal intensity over time, as well as dextran–FITC internalization throughout the PCT (white arrows) during its progressive morphogenesis. Double prkCI morphants displayed severe fluid accumulation, notably pericardial edema that remained strongly positive for the dextran–FITC conjugate label, and the PCT was not labeled by dextran endocytosis (left panels, lateral views; right panels, dorsal views, with exception of bottom right which shows a lateral/dorsal angled view).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
pax2a TU control Prim-25 pronephric tubule ISH
Protruding-mouth pronephric tubule ISH
TU + MO1-prkci + MO1-prkcz standard conditions Prim-25 pronephric podocyte ISH
Prim-25 pronephric proximal convoluted tubule ISH
Prim-25 pronephric tubule ISH
Protruding-mouth pronephric podocyte ISH
Protruding-mouth pronephric proximal convoluted tubule ISH
Protruding-mouth pronephric proximal straight tubule ISH
Protruding-mouth pronephric tubule ISH
podxl TU control Prim-25 pronephric podocyte ISH
Long-pec pronephric podocyte ISH
Protruding-mouth pronephric podocyte ISH
TU + MO1-prkci + MO1-prkcz standard conditions Prim-25 pronephric podocyte ISH
Long-pec pronephric podocyte ISH
Protruding-mouth pronephric podocyte ISH
Protruding-mouth pronephric proximal convoluted tubule ISH
wt1a TU control Prim-25 pronephric podocyte ISH
Long-pec pronephric podocyte ISH
Protruding-mouth pronephric podocyte ISH
TU + MO1-prkci + MO1-prkcz standard conditions Prim-25 pronephric podocyte ISH
Prim-25 pronephric proximal straight tubule ISH
Long-pec pronephric podocyte ISH
Long-pec pronephric proximal straight tubule ISH
Protruding-mouth pronephric podocyte ISH
Protruding-mouth pronephric proximal straight tubule ISH
wt1b TU control Prim-25 pronephric podocyte ISH
Long-pec pronephric podocyte ISH
Protruding-mouth pronephric podocyte ISH
TU + MO1-prkci + MO1-prkcz standard conditions Prim-25 pronephric podocyte ISH
Long-pec pronephric podocyte ISH
Protruding-mouth pronephric podocyte ISH
Protruding-mouth pronephric proximal convoluted tubule ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO1-prkci + MO1-prkcz standard conditions Prim-25 pronephric glomerulus development process quality, abnormal
TU + MO1-prkci + MO1-prkcz standard conditions Prim-25 pronephros pronephric nephron tubule epithelial cell differentiation process quality, abnormal
TU + MO1-prkci + MO1-prkcz standard conditions Long-pec pronephric glomerulus development process quality, abnormal
TU + MO1-prkci + MO1-prkcz standard conditions Long-pec pronephros pronephric nephron tubule epithelial cell differentiation process quality, abnormal
TU + MO1-prkci + MO1-prkcz standard conditions Protruding-mouth pronephric glomerulus development process quality, abnormal
TU + MO1-prkci + MO1-prkcz standard conditions Protruding-mouth pronephros pronephric nephron tubule epithelial cell differentiation process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 396(2), Gerlach, G.F., Wingert, R.A., Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta, 183-200, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.