ZFIN ID: ZDB-FIG-150309-9
Superina et al., 2014 - Analysis of maternal-zygotic ugdh mutants reveals divergent roles for HSPGs in vertebrate embryogenesis and provides new insight into the initiation of left-right asymmetry. Developmental Biology   387(2):154-166 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5

Fig. 4 Shh signaling is reduced in MZjek. (A,B) shh is expressed in the notochord and floorplate (arrowhead) of wildtype (A, N=30) and MZjek at 24 hpf (B, N=40). Insets show transverse sections of shh in the spinal cord (A',B' N=28,39). (C–H) Class II genes patched1, nkx2.2a, and olig2 are reduced in MZjek. patched1 expression is reduced in MZjek (D, N=26) as compared to wildtype embryos (C, N=18). Most notably, patched1 expression is severely reduced in the ZLI (arrowheads C,D). Insets show close up of head region, highlighting expression in the ZLI (arrowheads C′,D′). nkx2.2a is absent in prethalamic (anterior arrowhead) and thalamic (posterior arrowhead) areas surrounding the ZLI in MZjek mutants (E,E',F,F' N=25,37). olig2 expression is reduced in the forebrain (G,H arrowhead; N=12,17) and ventral spinal cord (arrow in insets G'H') in MZjek embryos. Boxed region in G,H shown at higher magnification in G′,H′. (I–L) Class I genes pax3a and dbx1a show increased expression in MZjek. pax3a expression is expanded in the dorsal spinal cord (bars) in MZjek embryos (I,I',J,J' N=23,34). Boxed region in I,J shown at higher magnification in I',J'. dbx1a expression is also expanded in the thalamic areas posterior to the ZLI (arrowheads K,K′,L,'' N=8,10).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dbx1a WT standard conditions Prim-5 spinal cord interneuron ISH
Prim-5 thalamus ISH
ugdhm310/m310 standard conditions Prim-5 spinal cord interneuron ISH
Prim-5 thalamus ISH
nkx2.2a WT standard conditions Prim-5 forebrain ventral region ISH
Prim-5 hindbrain ventral region ISH
Prim-5 midbrain ventral region ISH
Prim-5 spinal cord ISH
ugdhm310/m310 standard conditions Prim-5 forebrain ventral region ISH
Prim-5 hindbrain ventral region ISH
Prim-5 midbrain ventral region ISH
Prim-5 spinal cord ISH
olig2 WT standard conditions Prim-5 diencephalon ISH
Prim-5 spinal cord motor neuron ISH
ugdhm310/m310 standard conditions Prim-5 diencephalon ISH
Prim-5 spinal cord motor neuron ISH
pax3a WT standard conditions Prim-5 central nervous system dorsal region ISH
Prim-5 diencephalon ISH
Prim-5 hindbrain ISH
Prim-5 spinal cord dorsal-most region ISH
ugdhm310/m310 standard conditions Prim-5 central nervous system dorsal region ISH
Prim-5 diencephalon ISH
Prim-5 hindbrain ISH
Prim-5 spinal cord dorsal-most region ISH
ptch1 WT standard conditions Prim-5 neural tube ventral region ISH
Prim-5 somite ISH
Prim-5 zona limitans intrathalamica ISH
ugdhm310/m310 standard conditions Prim-5 neural tube ventral region ISH
Prim-5 somite ISH
Prim-5 zona limitans intrathalamica ISH
shha WT standard conditions Prim-5 medial floor plate ISH
Prim-5 notochord ISH
ugdhm310/m310 standard conditions Prim-5 medial floor plate ISH
Prim-5 notochord ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 387(2), Superina, S., Borovina, A., and Ciruna, B., Analysis of maternal-zygotic ugdh mutants reveals divergent roles for HSPGs in vertebrate embryogenesis and provides new insight into the initiation of left-right asymmetry, 154-166, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.