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ZFIN ID: ZDB-FIG-150309-14
Superina et al., 2014 - Analysis of maternal-zygotic ugdh mutants reveals divergent roles for HSPGs in vertebrate embryogenesis and provides new insight into the initiation of left-right asymmetry. Developmental Biology   387(2):154-166 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: 14-19 somites
PHENOTYPE:
Fish:
Observed In:
Stage: 10-13 somites

Fig. 9 Symmetry breaking in MZjek is abnormal downstream of flow establishment. (A,B) charon expression at KV in wildtype (A) and MZjek (B) embryos at 14 SS with corresponding ratios of left:right side expression quantification. (C) Ratio of left sided:right sided expression of charon surrounding KV in wildtype and MZjek (**p<0.01). (D,E) Confocal immunofluorescence of KV using anti-acetylated alpha-tubulin (green) and anti-phosphorylated CaMK-II (red) to visualize activated CaMK-II in cells surrounding the KV in wildtype (D) and MZjek (E) embryos at 12 SS (N=26, ***p<0.001).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dand5 WT standard conditions 14-19 somites Kupffer's vesicle ISH
ugdhm310/m310 standard conditions 14-19 somites Kupffer's vesicle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ugdhm310/m310 standard conditions 10-13 somites Kupffer's vesicle calcium-mediated signaling process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 387(2), Superina, S., Borovina, A., and Ciruna, B., Analysis of maternal-zygotic ugdh mutants reveals divergent roles for HSPGs in vertebrate embryogenesis and provides new insight into the initiation of left-right asymmetry, 154-166, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.