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ZFIN ID: ZDB-FIG-140422-69
Superina et al., 2014 - Analysis of maternal-zygotic ugdh mutants reveals divergent roles for HSPGs in vertebrate embryogenesis and provides new insight into the initiation of left-right asymmetry. Developmental Biology   387(2):154-166 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Term:
Stage: Shield
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 1

MZjek mutant embryos lack extracellular heparan sulfate proteoglycans and display multiple morphological abnormalities. (A,B) Representative confocal micrographs of anti- heparan sulfate PG staining in the animal pole of shield-staged wildtype (A) and MZjek mutant (B) embryos (N=20,20 respectively). Anti-heparan sulfate staining is clearly present in the extracellular space of wildtype embryos. In MZjek, there is an absence of HS staining, suggesting HS GAGs are not synthesized in the mutant. (C,D) Live images of 48 hpf WT (C) and MZjek mutant embryos (D) mounted for lateral, dorsal and ventral views. MZjek (D) exhibit an expanded mediolateral axis (bars), defects in axial extension, brain patterning defects (arrowheads) and disrupted heart morphogenesis.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab-10E4 IHC WT standard conditions Shield anatomical structure extracellular space
IHC ugdhm310/m310 standard conditions Shield Not Detected anatomical structure extracellular space
Phenotype Details
Fish Conditions Stage Phenotype
ugdhm310/m310 standard conditions Long-pec caudal fin decreased size, abnormal
ugdhm310/m310 standard conditions Long-pec central nervous system morphology, abnormal
ugdhm310/m310 standard conditions Long-pec central nervous system development process quality, abnormal
ugdhm310/m310 standard conditions Long-pec heart malformed, abnormal
ugdhm310/m310 standard conditions Long-pec heart development process quality, abnormal
ugdhm310/m310 standard conditions Long-pec whole organism decreased length, abnormal
ugdhm310/m310 standard conditions Long-pec whole organism increased width, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 387(2), Superina, S., Borovina, A., and Ciruna, B., Analysis of maternal-zygotic ugdh mutants reveals divergent roles for HSPGs in vertebrate embryogenesis and provides new insight into the initiation of left-right asymmetry, 154-166, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.