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ZFIN ID: ZDB-FIG-150309-11
Superina et al., 2014 - Analysis of maternal-zygotic ugdh mutants reveals divergent roles for HSPGs in vertebrate embryogenesis and provides new insight into the initiation of left-right asymmetry. Developmental Biology   387(2):154-166 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Shield to 10-13 somites
PHENOTYPE:
Fish:
Observed In:
Stage Range: Shield to 10-13 somites

Fig. 6 Canonical Wnt signaling is upregulated in MZjek;. (A–F) Whole-mount in situ hybridization (WISH) for dorsal organizer genes goosecoid (A,B; gsc; N=26,23) and chordin (C,D; chd; N=20,21), and ventral gene vox (E,F; N=15,18) at shield stage. Dorsal (D) and Ventral (V) orientation indicated in panel A. Embryos are viewed from the animal pole with dorsal to the right. (G–L) WISH of axin2 (G,H; N=12,8), sp5 (I,J; N=18,11), and sp5l (K,L; N=9,8) at 12 SS. Gene expression appears to be most dramatically upregulated in the tailbud (arrowheads). Anterior (A) and Posterior (P) extents are as indicated in panel G.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
axin2 WT standard conditions 10-13 somites anatomical structure ISH
10-13 somites tail bud ISH
ugdhm310/m310 standard conditions 10-13 somites anatomical structure ISH
10-13 somites tail bud ISH
chrd WT standard conditions Shield shield ISH
ugdhm310/m310 standard conditions Shield shield ISH
gsc WT standard conditions Shield shield ISH
ugdhm310/m310 standard conditions Shield shield ISH
sp5a WT standard conditions 10-13 somites anatomical structure ISH
10-13 somites tail bud ISH
ugdhm310/m310 standard conditions 10-13 somites anatomical structure ISH
10-13 somites tail bud ISH
sp5l WT standard conditions 10-13 somites anatomical structure ISH
10-13 somites tail bud ISH
ugdhm310/m310 standard conditions 10-13 somites anatomical structure ISH
10-13 somites tail bud ISH
vox WT standard conditions Shield whole organism ventral region ISH
ugdhm310/m310 standard conditions Shield whole organism ventral region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ugdhm310/m310 standard conditions Shield canonical Wnt signaling pathway increased process quality, abnormal
10-13 somites canonical Wnt signaling pathway increased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 387(2), Superina, S., Borovina, A., and Ciruna, B., Analysis of maternal-zygotic ugdh mutants reveals divergent roles for HSPGs in vertebrate embryogenesis and provides new insight into the initiation of left-right asymmetry, 154-166, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.