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ZFIN ID: ZDB-FIG-150309-12
Superina et al., 2014 - Analysis of maternal-zygotic ugdh mutants reveals divergent roles for HSPGs in vertebrate embryogenesis and provides new insight into the initiation of left-right asymmetry. Developmental Biology   387(2):154-166 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: 20-25 somites
PHENOTYPE:
Fish:
Observed In:
Stage: 20-25 somites

Fig. 7 MZjek mutants show left–right patterning defects despite having intact embryonic midlines. (A–D) WISH for lefty1,2 (A,C; N=80,116) and spaw (B,D; N=16,43) in wildtype and MZjek embryos at 21 SS. (E) Percentage of embryos displaying left, right, bilateral and absent lefty1,2 and spaw expression in 20–22 SS wildtype (wt) and MZjek mutant embryos. (F–K) Midline markers shh (F,I), ntl (G,J), and lefty1 (H,K) are expressed continuously along the embryonic axis in both wildtype and MZjek embryos, indicating an intact midline.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
lft1 WT standard conditions 20-25 somites lateral plate mesoderm left side ISH
ugdhm310/m310 standard conditions 20-25 somites lateral plate mesoderm ISH
20-25 somites lateral plate mesoderm left side ISH
20-25 somites lateral plate mesoderm right side ISH
lft2 WT standard conditions 20-25 somites lateral plate mesoderm left side ISH
ugdhm310/m310 standard conditions 20-25 somites lateral plate mesoderm ISH
20-25 somites lateral plate mesoderm left side ISH
20-25 somites lateral plate mesoderm right side ISH
shha WT standard conditions 20-25 somites floor plate ISH
20-25 somites notochord ISH
ugdhm310/m310 standard conditions 20-25 somites floor plate ISH
20-25 somites notochord ISH
spaw WT standard conditions 20-25 somites lateral plate mesoderm left side ISH
ugdhm310/m310 standard conditions 20-25 somites lateral plate mesoderm ISH
20-25 somites lateral plate mesoderm left side ISH
20-25 somites lateral plate mesoderm right side ISH
tbxta WT standard conditions 20-25 somites notochord ISH
ugdhm310/m310 standard conditions 20-25 somites notochord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ugdhm310/m310 standard conditions 20-25 somites determination of left/right asymmetry in lateral mesoderm process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 387(2), Superina, S., Borovina, A., and Ciruna, B., Analysis of maternal-zygotic ugdh mutants reveals divergent roles for HSPGs in vertebrate embryogenesis and provides new insight into the initiation of left-right asymmetry, 154-166, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.