header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-150309-10
Superina et al., 2014 - Analysis of maternal-zygotic ugdh mutants reveals divergent roles for HSPGs in vertebrate embryogenesis and provides new insight into the initiation of left-right asymmetry. Developmental Biology   387(2):154-166 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: 10-13 somites
PHENOTYPE:
Fish:
Observed In:
Stage: 5-9 somites

Fig. 5 Convergence and extension defects are apparent in MZjek. (A,B) Representative photos of live wildtype (A; N=9) and MZjek (B; N=7) embryos at 9 SS. (C) Quantification of the angle of body axis extension over the yolk in wildtype, MZjek and Zkny (***p<0.001). (D,E) Flat-mounts of 10 SS wildtype (D; N=13) and MZjek (E; N=10) embryos stained for krox-20 (hindbrain) and myoD (somite) gene expression. (F) Quantification of the anterior–posterior extent of the myoD expression domain in wildtype versus MZjek (***p<0.001). (G) Quantification of the mediolateral extent of krox20 expression in wildtype versus MZjek (***p<0.001).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
egr2b WT standard conditions 10-13 somites hindbrain ISH
ugdhm310/m310 standard conditions 10-13 somites hindbrain ISH
myod1 WT standard conditions 10-13 somites somite ISH
ugdhm310/m310 standard conditions 10-13 somites somite ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ugdhm310/m310 standard conditions 5-9 somites convergent extension process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 387(2), Superina, S., Borovina, A., and Ciruna, B., Analysis of maternal-zygotic ugdh mutants reveals divergent roles for HSPGs in vertebrate embryogenesis and provides new insight into the initiation of left-right asymmetry, 154-166, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.