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ZFIN ID: ZDB-FIG-150309-8
Superina et al., 2014 - Analysis of maternal-zygotic ugdh mutants reveals divergent roles for HSPGs in vertebrate embryogenesis and provides new insight into the initiation of left-right asymmetry. Developmental Biology   387(2):154-166 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Fig. 3 Fgf signaling is reduced in MZjek. (A–F) WISH for fgf8, spry4 and pea3 in wildtype (A,C,E; tailbud out of frame) and MZjek (B,D,F) embryos. In MZjek, fgf8 is present and expressed at equivalent levels to wildtype embryos (A,B; N=62,60), however targets spry4 (C,D; N=92,75) and pea3 (E,F; N=35,39) appear reduced. (G) qRT-PCR assays of fgf8 (p=0.2257), spry4 (*p<0.05), pea3 (**p<0.01) expression in wildtype versus MZjek embryos at 24 hpf. Error bars represent the s.e. for the expression level fold change.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
etv4 WT standard conditions Prim-5 midbrain hindbrain boundary ISH
ugdhm310/m310 standard conditions Prim-5 midbrain hindbrain boundary ISH
fgf8a WT standard conditions Prim-5 midbrain hindbrain boundary ISH
ugdhm310/m310 standard conditions Prim-5 midbrain hindbrain boundary ISH
spry4 WT standard conditions Prim-5 midbrain hindbrain boundary ISH
ugdhm310/m310 standard conditions Prim-5 midbrain hindbrain boundary ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ugdhm310/m310 standard conditions Prim-5 fibroblast growth factor receptor signaling pathway decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 387(2), Superina, S., Borovina, A., and Ciruna, B., Analysis of maternal-zygotic ugdh mutants reveals divergent roles for HSPGs in vertebrate embryogenesis and provides new insight into the initiation of left-right asymmetry, 154-166, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.