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ZFIN ID: ZDB-FIG-140422-70
Superina et al., 2014 - Analysis of maternal-zygotic ugdh mutants reveals divergent roles for HSPGs in vertebrate embryogenesis and provides new insight into the initiation of left-right asymmetry. Developmental Biology   387(2):154-166 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: 20-25 somites to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 2

MZjek have a dysmorphic heart and reduced ventricular populations. (A,B) Lateral views of hearts stained with MF20 (red) and S46 (green) antibodies to visualize the ventricle and atrium of 48 hpf embryos. MF20 marks the entire heart and S46 is atrium specific. In these superimposed images MF20+S46- ventricle is red and MF20+S46+ atrium appears yellow. (A) Wildtype heart with two distinct chambers. (B) MZjek display a dysmorphic heart, with a misshapen atrium and a strung out ventricle (N=9). (C–F) amhc and vmhc expression in wildtype (C,E) and MZjek (D,F) at the 20-somite stage. MZjek mutants exhibit reduced expression of vmhc when compared to similarly staged wildtype embryos (N=25, 36 respectively).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myh6 WT standard conditions 20-25 somites heart rudiment ISH
ugdhm310/m310 standard conditions 20-25 somites heart rudiment ISH
myh7 WT standard conditions 20-25 somites heart rudiment ISH
ugdhm310/m310 standard conditions 20-25 somites heart rudiment ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-MF20 IHC WT standard conditions Long-pec heart
IHC ugdhm310/m310 standard conditions Long-pec heart
Ab-S46 IHC WT standard conditions Long-pec atrium
IHC ugdhm310/m310 standard conditions Long-pec atrium
Phenotype Details
Fish Conditions Stage Phenotype
ugdhm310/m310 standard conditions Long-pec atrium shape, abnormal
ugdhm310/m310 standard conditions Long-pec cardiac ventricle morphology, abnormal
ugdhm310/m310 standard conditions Long-pec cardiac ventricle shape, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 387(2), Superina, S., Borovina, A., and Ciruna, B., Analysis of maternal-zygotic ugdh mutants reveals divergent roles for HSPGs in vertebrate embryogenesis and provides new insight into the initiation of left-right asymmetry, 154-166, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.