ZFIN ID: ZDB-FIG-090527-13
Vitorino et al., 2009 - Vsx2 in the zebrafish retina: restricted lineages through derepression. Neural Development   4:14 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage Range: Long-pec to Protruding-mouth

Fig. 12 Transcription factors that are upregulated and downregulated in Vsx2 morphants. RT-PCR of control (Ctrl) and Vsx2 morphant embryos showing changes in gene expression. mRNAs for Vsx2, Ath5, Mitf, and Foxn4 are all upregulated in Vsx2 morphants, suggesting that these transcription factors are normally repressed by Vsx2. On the other hand, Crx, Bhlh4 and Rx3 are downregulated in Vsx2 morphants, suggesting that these genes are indirectly positively controlled by Vsx2. Vsx1 levels seem to be unchanged in Vsx2 morphants. Vsx2e1-e2 (the PCR product of primers that amplify the Vsx2 region from exon 1 to exon 2) is also upregulated when Vsx2 is knocked down with the translational morpholino (mo), whilst efficiency of the splicing morpholinos (smo) can be seen by the increased PCR product size due to splicing being prevented by the morpholino. The housekeeping gene encoding EF1α (Elongation factor 1α) was used as a loading control.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
atoh7 WT control Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT + MO2-vsx2 standard conditions Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT + MO3-vsx2 + MO4-vsx2 standard conditions Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
bhlhe23 WT control Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT + MO2-vsx2 standard conditions Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT + MO3-vsx2 + MO4-vsx2 standard conditions Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
crx WT control Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT + MO2-vsx2 standard conditions Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT + MO3-vsx2 + MO4-vsx2 standard conditions Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
eef1a1l1 WT control Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT + MO2-vsx2 standard conditions Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT + MO3-vsx2 + MO4-vsx2 standard conditions Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
foxn4 WT control Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT + MO2-vsx2 standard conditions Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT + MO3-vsx2 + MO4-vsx2 standard conditions Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
mitfa WT control Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT + MO2-vsx2 standard conditions Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT + MO3-vsx2 + MO4-vsx2 standard conditions Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
rx3 WT control Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT + MO2-vsx2 standard conditions Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT + MO3-vsx2 + MO4-vsx2 standard conditions Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
vsx1 WT control Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT + MO2-vsx2 standard conditions Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT + MO3-vsx2 + MO4-vsx2 standard conditions Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
vsx2 WT control Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT + MO2-vsx2 standard conditions Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT + MO3-vsx2 + MO4-vsx2 standard conditions Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Neural Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neural Dev.