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ZFIN ID: ZDB-FIG-090527-10
Vitorino et al., 2009 - Vsx2 in the zebrafish retina: restricted lineages through derepression. Neural Development   4:14 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to High-pec

Fig. 9 Ath5 progenitor cells arise from Vsx2 progenitors that lose Vsx2:GFP expression. (A, B) Images of live double transgenic Tg(vsx2:GFP/ath5:GapRFP) whole-mount retinas at different time points of retina development indicated as hours post-fertilisation (hpf). (A) At 28 hpf, a few cells in the retina no longer express green fluorescent protein (GFP) driven by vsx2. Some (white arrows), but not all of these Vsx2:GFP-negative cells express Ath5:RFP. (A′-B′) As development progresses, the number of cells that express Ath5:RFP increase as the number of Vsx2:GFP cells diminishes. Ath5-expressing, Vsx2:GFP-negative cells divide apically and many of the differentiating cells can be seen to settle in the developing ganglion cell layer, which becomes devoid of Vsx2:GFP cells. Images are from the movie in Additional data file 5. (C-F) Double label from a Tg(ath5:RFP;vsx2:GFP) retina in which an Ath5:RFP progenitor divides once to produce one daughter that differentiates as an RGC. (E′-F′) Vsx2 expression can be seen to be downregulated in this Ath5:RFP progenitor (whose soma is marked by the white dot). Scale bars: (A) 16 μm; (B) 27 μm; (C-F) 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP cu2Tg; nns1Tg standard conditions Prim-5 optic cup IFL
High-pec retina IFL
RFP cu2Tg; nns1Tg standard conditions Prim-5 optic cup IFL
High-pec retina IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Neural Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neural Dev.