ZFIN ID: ZDB-FIG-090527-14
Vitorino et al., 2009 - Vsx2 in the zebrafish retina: restricted lineages through derepression. Neural Development   4:14 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 4
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 13 Loss of Vsx2 allows various cell types to develop in the retina. (A-J) Cryosections of 80 hours post-fertilization (hpf) retinas labelled with different cell-specific markers show that Vsx2 morphant retinas have all cell types present: Sox2 labels amacrine and Müller cells (A, B); protein kinase C (PKC)β1 labels some bipolar cells (C, D); Hermes labels ganglion cells (E, F); Islet 1 labels horizontal, bipolar, some amacrine and ganglion cells (G, H); glutamine synthetase (GS) labels Müller cells (I, J). (K-T)Cryostat sections of 80 hpf transgenic retinas similarly reveal presence of all marked cell types in Vsx2 morphants. (K-N) Vsx2 morphant Tg(ath5:Gap-GFP) retinas (double labelled with the ganglion cell marker Zn5 in red) still have green fluorescent protein (GFP)- and Zn5-labelled ganglion cells and form an optic nerve to the tectum. (O-R) Vsx2 morphant double transgenic Tg(vsx1:GFP-vsx2:dsRed) have both GFP- and DsRed-labelled cells, but appear to have comparatively fewer Vsx2:DsRed cells. As in the control transgenics, Vsx1:GFP and Vsx2:DsRed do not co-localise in the same cells. (S, T) Vsx2 morphant Tg(ptf1a:GFP) retinas show GFP labelling for horizontal and amacrine similar to that in controls, although some GFP cells are displaced and can be found closer to the lens in the GCL of the morphant retinas. L, lens. Scale bar: (A-T) 47 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed nns3Tg; nns5Tg standard conditions Protruding-mouth retinal inner nuclear layer retinal bipolar neuron IFL
nns3Tg; nns5Tg + MO2-vsx2 standard conditions Protruding-mouth retinal inner nuclear layer retinal bipolar neuron IFL
nns3Tg; nns5Tg + MO3-vsx2 + MO4-vsx2 standard conditions Protruding-mouth retinal inner nuclear layer retinal bipolar neuron IFL
EGFP cu1Tg control Protruding-mouth cranial nerve II IFL
Protruding-mouth retinal ganglion cell IFL
Day 4 cranial nerve II IFL
Day 4 retinal ganglion cell IFL
cu1Tg + MO2-vsx2 standard conditions Protruding-mouth cranial nerve II IFL
Protruding-mouth retinal ganglion cell IFL
Day 4 cranial nerve II IFL
Day 4 retinal ganglion cell IFL
cu1Tg + MO3-vsx2 + MO4-vsx2 standard conditions Protruding-mouth cranial nerve II IFL
Protruding-mouth retinal ganglion cell IFL
Day 4 cranial nerve II IFL
Day 4 retinal ganglion cell IFL
jh1Tg standard conditions Protruding-mouth amacrine cell IFL
Protruding-mouth horizontal cell IFL
jh1Tg + MO2-vsx2 standard conditions Protruding-mouth amacrine cell IFL
Protruding-mouth horizontal cell IFL
jh1Tg + MO3-vsx2 + MO4-vsx2 standard conditions Protruding-mouth amacrine cell IFL
Protruding-mouth horizontal cell IFL
nns3Tg; nns5Tg standard conditions Protruding-mouth retinal inner nuclear layer retinal bipolar neuron IFL
nns3Tg; nns5Tg + MO2-vsx2 standard conditions Protruding-mouth retinal inner nuclear layer retinal bipolar neuron IFL
nns3Tg; nns5Tg + MO3-vsx2 + MO4-vsx2 standard conditions Protruding-mouth retinal inner nuclear layer retinal bipolar neuron IFL
hem WT control Protruding-mouth retinal ganglion cell IHC
WT + MO2-vsx2 standard conditions Protruding-mouth retinal ganglion cell IHC
WT + MO3-vsx2 + MO4-vsx2 standard conditions Protruding-mouth retinal ganglion cell IHC
isl1 WT control Protruding-mouth amacrine cell ISH
Protruding-mouth horizontal cell ISH
Protruding-mouth retinal bipolar neuron ISH
Protruding-mouth retinal ganglion cell ISH
WT + MO2-vsx2 standard conditions Protruding-mouth amacrine cell ISH
Protruding-mouth horizontal cell ISH
Protruding-mouth retinal bipolar neuron ISH
Protruding-mouth retinal ganglion cell ISH
WT + MO3-vsx2 + MO4-vsx2 standard conditions Protruding-mouth amacrine cell ISH
Protruding-mouth horizontal cell ISH
Protruding-mouth retinal bipolar neuron ISH
Protruding-mouth retinal ganglion cell ISH
rbpms2b Ab1-rbpms2 WT control Protruding-mouth retinal ganglion cell IHC
Ab1-rbpms2 WT + MO2-vsx2 standard conditions Protruding-mouth retinal ganglion cell IHC
Ab1-rbpms2 WT + MO3-vsx2 + MO4-vsx2 standard conditions Protruding-mouth retinal ganglion cell IHC
sox2 Ab1-sox2 WT control Protruding-mouth amacrine cell IHC
Ab1-sox2 Protruding-mouth Muller cell IHC
Ab1-sox2 WT + MO2-vsx2 standard conditions Protruding-mouth amacrine cell IHC
Ab1-sox2 Protruding-mouth Muller cell IHC
Ab1-sox2 WT + MO3-vsx2 + MO4-vsx2 standard conditions Protruding-mouth amacrine cell IHC
Ab1-sox2 Protruding-mouth Muller cell IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nns3Tg; nns5Tg + MO2-vsx2 standard conditions Protruding-mouth Muller cell decreased amount, abnormal
Protruding-mouth retinal bipolar neuron decreased amount, abnormal
nns3Tg; nns5Tg + MO3-vsx2 + MO4-vsx2 standard conditions Protruding-mouth Muller cell decreased amount, abnormal
Protruding-mouth retinal bipolar neuron decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Neural Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neural Dev.