header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-PUB-090407-16
Vsx2 in the zebrafish retina: restricted lineages through derepression
Vitorino, M., Jusuf, P.R., Maurus, D., Yukiko, Y., Higashijima, S.I., and Harris, W.A.
Date: 2009
Source: Neural Development 4: 14 (Journal)
Registered Authors: Harris, William A., Higashijima, Shin-ichi, Jusuf, Patricia
Keywords: none
MeSH Terms:
  • Amacrine Cells/cytology
  • Amacrine Cells/metabolism
  • Animals
  • Cell Differentiation/genetics
  • Cell Lineage/genetics
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • Down-Regulation/genetics
  • Eye Proteins/genetics
  • Eye Proteins/metabolism*
  • Forkhead Transcription Factors/genetics
  • Forkhead Transcription Factors/metabolism
  • Gene Expression Regulation, Developmental/genetics
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism*
  • Neurogenesis/genetics
  • Neuroglia/cytology
  • Neuroglia/metabolism
  • Photoreceptor Cells, Vertebrate/cytology
  • Photoreceptor Cells, Vertebrate/metabolism
  • Repressor Proteins/genetics
  • Repressor Proteins/metabolism
  • Retina/cytology
  • Retina/embryology*
  • Retina/metabolism*
  • Retinal Bipolar Cells/cytology
  • Retinal Bipolar Cells/metabolism
  • Retinal Ganglion Cells/cytology
  • Retinal Ganglion Cells/metabolism
  • Retinal Horizontal Cells/cytology
  • Retinal Horizontal Cells/metabolism
  • Stem Cells/cytology
  • Stem Cells/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 19344499 Full text @ Neural Dev.
FIGURES
ABSTRACT
BACKGROUND: The neurons in the vertebrate retina arise from multipotent retinal progenitor cells (RPCs). It is not clear, however, which progenitors are multipotent or why they are multipotent. RESULTS: In this study we show that the homeodomain transcription factor Vsx2 is initially expressed throughout the retinal epithelium, but later it is downregulated in all but a minor population of bipolar cells and all Muller glia. The Vsx2-negative daughters of Vsx2-positive RPCs divide and give rise to all other cell types in the retina. Vsx2 is a repressor whose targets include transcription factors such as Vsx1, which is expressed in the progenitors of distinct non-Vsx2 bipolars, and the basic helix-loop-helix transcription factor Ath5, which restricts the fate of progenitors to retinal ganglion cells, horizontal cells, amacrine cells and photoreceptors fates. Foxn4, expressed in the progenitors of amacrine and horizontal cells, is also negatively regulated by Vsx2. CONCLUSIONS: Our data thus suggest Vsx2-positive RPCs are fully multipotent retinal progenitors and that when Vsx2 is downregulated, Vsx2-negative progenitors escape Vsx2 repression and so are able to express factors that restrict lineage potential.
ADDITIONAL INFORMATION